Invitrogen

Bax Monoclonal Antibody (SP47)

1 Reference
View all (99) BAX antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-16322

Applications
Tested Dilution
Publications

Western Blot (WB)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50
-
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

SP47

Immunogen

Synthetic peptide corresponding to internal region of human BAX protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Protein A/G

Storage buffer

PBS, pH 7.6, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537841

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is normal breast tissue.

Target Information

BAX is a members of the Bcl-2 Family and plays an important role in regulation of apoptosis. Whereas Bcl-2 is commonly regarded as an anti-apoptotic protein, BAX is considered to have a pro-apoptotic function. Regulation of apoptosis is supposed to involve both homo- and heterodimerization of different isoforms of BAX and Bcl-2. The Bax gene encodes different isoforms including Bax alpha (21 kDa) and Bax beta (24 kDa), whereas both isoforms contain the BH1, BH2 and BH3 domains, Bax beta has a unique carboxyl terminus and does not contain a hydrophobic transmembrane domain. Bcl-2 is also expressed in different Isoforms. Bcl-2 beta differs in the 3' UTR and coding region compared to variant alpha. Bcl-2 beta is shorter (22 kDa) and has a distinct C-terminus compared to Bcl-2 alpha (26 kDa). BAX is a member of the BCL-2 family of proteins, which function as regulators of apoptosis. Overexpression of BAX functions to promote cell death. BAX can form homodimers and is also able to heterodimerize with other BCL-2 related proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Apoptosis regulator BAX; Bax zeta; Baxdelta2G9; Baxdelta2G9omega; Baxdelta2omega; Bcl-2-like protein 4; BCL2 associated X protein; BCL2-associated X protein omega; Bcl2-L-4

View more View less

Gene Aliases: BAX; BCL2L4

View more View less

UniProt ID: (Human) Q07812

View more View less

Entrez Gene ID: (Human) 581

View more View less

Function(s)
protein binding lipid binding channel activity heat shock protein binding protein complex binding identical protein binding protein homodimerization activity protein heterodimerization activity chaperone binding BH3 domain binding
Process(es)
ovarian follicle development neuron migration T cell homeostatic proliferation B cell homeostasis B cell apoptotic process kidney development release of cytochrome c from mitochondria protein insertion into mitochondrial membrane involved in apoptotic signaling pathway blood vessel remodeling myeloid cell homeostasis B cell negative selection B cell homeostatic proliferation positive regulation of B cell apoptotic process glycosphingolipid metabolic process regulation of nitrogen utilization apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest activation of signaling protein activity involved in unfolded protein response inner mitochondrial membrane organization outer mitochondrial membrane organization germ cell development aging mitochondrial fusion extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c apoptotic mitochondrial changes fertilization response to toxic substance response to salt stress establishment or maintenance of transmembrane electrochemical gradient response to gamma radiation viral process hypothalamus development cerebral cortex development negative regulation of protein binding positive regulation of protein oligomerization endoplasmic reticulum calcium ion homeostasis negative regulation of endoplasmic reticulum calcium ion concentration release of matrix enzymes from mitochondria negative regulation of peptidyl-serine phosphorylation regulation of mammary gland epithelial cell proliferation glial cell apoptotic process cellular response to UV ectopic germ cell programmed cell death response to cocaine odontogenesis of dentin-containing tooth response to drug regulation of apoptotic process positive regulation of apoptotic process regulation of protein homodimerization activity regulation of protein heterodimerization activity negative regulation of neuron apoptotic process positive regulation of neuron apoptotic process mitochondrial fragmentation involved in apoptotic process development of secondary sexual characteristics cellular respiration retinal cell programmed cell death response to copper ion positive regulation of developmental pigmentation negative regulation of fibroblast proliferation spermatid differentiation post-embryonic camera-type eye morphogenesis response to axon injury homeostasis of number of cells within a tissue protein oligomerization protein homooligomerization positive regulation of release of sequestered calcium ion into cytosol neuron apoptotic process response to corticosterone regulation of mitochondrial membrane potential Sertoli cell proliferation retina development in camera-type eye positive regulation of apoptotic process involved in mammary gland involution vagina development intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress thymocyte apoptotic process mitochondrion morphogenesis intrinsic apoptotic signaling pathway by p53 class mediator positive regulation of release of cytochrome c from mitochondria apoptotic signaling pathway extrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand intrinsic apoptotic signaling pathway activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway positive regulation of endoplasmic reticulum unfolded protein response positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway apoptotic process involved in patterning of blood vessels apoptotic process involved in embryonic digit morphogenesis regulation of mitochondrial membrane permeability involved in programmed necrotic cell death positive regulation of apoptotic DNA fragmentation positive regulation of IRE1-mediated unfolded protein response B cell receptor apoptotic signaling pathway negative regulation of apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway in absence of ligand positive regulation of intrinsic apoptotic signaling pathway
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support