Invitrogen

Basophil Marker Monoclonal Antibody (2D7), PE, eBioscience™

Basophil Marker Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

Basophil Marker Antibody (12-9748-42) in Flow

Normal human peripheral blood cells were surface stained with Anti-Human CD123 APC (Product # 17-1239). The cells were then permeabilized with the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824), and intracellularly stained with Mouse IgG1 K Isotype Control PE (Product # 12-4714) (left) or Anti-Human Basophil Marker PE (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

12-9748-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2D7

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572730

Product Specific Information

Description: The 2D7 monoclonal antibody recognizes human 2D7 antigen expressed exclusively by human basophils. This human basophil marker is a 7.2-7.5 kDa protein present exclusively in the secretory granules of human basophils. Upon activation, basophils may degranulate, thereby decreasing the amount of 2D7 antigen available for staining. This antibody does not stain mast cells.

Applications Reported: This 2D7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 2D7 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This antibody can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The human 2D7 antigen is expressed exclusively by human basophils. This human basophil marker is a 7.2-7.5 kDa protein present exclusively in the secretory granules of human basophils. Upon activation, basophils may degranulate, thereby decreasing the amount of 2D7 antigen available for staining.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Bioinformatics

Protein Aliases: Basophil 2D7 marker; Basophils

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