Invitrogen

BrdU Monoclonal Antibody (MoBU-1), Alexa Fluor™ 488

FIGURE: 1 / 13

BrdU Antibody (B35139) in Flow

Jurkat T-cell leukemia cells were treated with 10µM BrdU for 1.5 hours. The cells were then fixed in ETOH and stored overnight at =-20°C. An acid denaturation method was used to prepare the cells before labeling with BrdU mouse monoclonal antibody (Clone MoBU-1) Alexa Fluor® 488 *for flow cytometry* *100 tests* to detect the incorporated BrdU, each using 5 µL antibody conjugate labeling 1 x 10^6 cells. Proliferating cells are clearly distinguished from non-proliferating cells.

BrdU Antibody in Immunocytochemistry (ICC/IF)

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Product Details

B35139

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

Assay-dependent

View 9 publications 9 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chemical

Published species

Chemical

Host/Isotype

Mouse / IgG

Class

Monoclonal

Type

Antibody

Clone

MoBU-1

Immunogen

BrdU

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

5mM sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2536439

Target Information

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

A multistate stem cell dynamics maintains homeostasis in mouse spermatogenesis.

Cell reports

Nakagawa T,Jörg DJ,Watanabe H,Mizuno S,Han S,Ikeda T,Omatsu Y,Nishimura K,Fujita M,Takahashi S,Kondoh G,Simons BD,Yoshida S,Nagasawa T

B35139 was used in Immunohistochemistry to investigate if an ensemble of heterogeneous states of spermatogenic stem cell supports homeostatic, persistent spermatogenesis.

Tue Oct 19 00:00:00 EDT 2021

c-Myb Exacerbates Atherosclerosis through Regulation of Protective IgM-Producing Antibody-Secreting Cells.

Cell reports

Shikatani EA,Besla R,Ensan S,Upadhye A,Khyzha N,Li A,Emoto T,Chiu F,Degousee N,Moreau JM,Perry HM,Thayaparan D,Cheng HS,Pacheco S,Smyth D,Noyan H,Zavitz CCJ,Bauer CMT,Hilgendorf I,Libby P,Swirski FK,Gommerman JL,Fish JE,Stampfli MR,Cybulsky MI,Rubin BB,Paige CJ,Bender TP,McNamara CA,Husain M,Robbins CS

B35139 was used in Flow Cytometry to identify the nuclear transcription factor c-Myb as an important mediator of atherosclerotic disease in mice.

Tue May 21 00:00:00 EDT 2019

Fluorescence-based assay for accurate measurement of transcriptional activity in trypanosomatid parasites.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Hiraiwa PM,de Aguiar AM,Ávila AR

B35139 was used in Flow cytometry/Cell sorting to contribute to obtaining precise measurements of transcriptional rates using multiparametric analysis.

Sun Jul 01 00:00:00 EDT 2018

Multiscale cytometry and regulation of 3D cell cultures on a chip.

Nature communications

Sart S,Tomasi RF,Amselem G,Baroud CN

Published figure using BrdU monoclonal antibody (Product # B35139) in Immunofluorescence

Thu Sep 07 00:00:00 EDT 2017

YAP regulates S-phase entry in endothelial cells.

PloS one

Shen Z,Stanger BZ

B35139 was used in flow cytometry to study the role of YAP in cell proliferation and survival using different cell types

Sat Jan 30 00:00:00 EST 2016

YAP regulates S-phase entry in endothelial cells.

PloS one

Shen Z,Stanger BZ

B35139 was used in flow cytometry to study the role of YAP in cell proliferation and survival using different cell types

Sat Jan 30 00:00:00 EST 2016

The Fanconi Anemia Pathway Protects Genome Integrity from R-loops.

PLoS genetics

García-Rubio ML,Pérez-Calero C,Barroso SI,Tumini E,Herrera-Moyano E,Rosado IV,Aguilera A

B35139 was used in flow cytometry to elucidate how genome integrity is protected from R-loops in the fanconi anemia pathway

Sun Nov 01 00:00:00 EDT 2015

Basal p21 controls population heterogeneity in cycling and quiescent cell cycle states.

Proceedings of the National Academy of Sciences of the United States of America

Overton KW,Spencer SL,Noderer WL,Meyer T,Wang CL

B35139 was used in flow cytometry to analyze how population heterogeneity in quiescent and cycling cell cycle states is controlled by basal p21

Tue Oct 14 00:00:00 EDT 2014

Tumor suppressor and deubiquitinase BAP1 promotes DNA double-strand break repair.

Proceedings of the National Academy of Sciences of the United States of America

Yu H,Pak H,Hammond-Martel I,Ghram M,Rodrigue A,Daou S,Barbour H,Corbeil L,Hébert J,Drobetsky E,Masson JY,Di Noia JM,Affar el B

B35139 was used in flow cytometry to determine deubiquitinases required to assemble homologous recombination factors BRCA1 and RAD51

Tue Jan 07 00:00:00 EST 2014

Wild-type p53-induced phosphatase 1 (Wip1) forestalls cellular premature senescence at physiological oxygen levels by regulating DNA damage response signaling during DNA replication.

Cell cycle (Georgetown, Tex.)

Sakai H,Fujigaki H,Mazur SJ,Appella E

Published figure using BrdU monoclonal antibody (Product # B35139) in Flow Cytometry

Wed Oct 22 00:00:00 EDT 2014

Thermally targeted p21 peptide enhances bortezomib cytotoxicity in androgen-independent prostate cancer cell lines.

Anti-cancer drugs

Mikecin AM,Walker LR,Kuna M,Raucher D

B35139 was used in flow cytometry to examine the combination treatment of prostate cancer cells with bortezomib and the C-terminal part of the p21(WAF1/CIP1) protein bound to the Elastin-like polypeptide carrier.

Sat Feb 01 00:00:00 EST 2014

Upregulation of miR-96 enhances cellular proliferation of prostate cancer cells through FOXO1.

PloS one

Haflidadóttir BS,Larne O,Martin M,Persson M,Edsjö A,Bjartell A,Ceder Y

B35139 was used in flow cytometry to examine the diagnostic and prognostic properties of miR-96 expression levels in prostate cancer cohorts.

Tue Apr 15 00:00:00 EDT 2014

Dual-pulse labeling using 5-ethynyl-2'-deoxyuridine (EdU) and 5-bromo-2'-deoxyuridine (BrdU) in flow cytometry.

Current protocols in cytometry

Bradford JA,Clarke ST

B35139 was used in Flow Cytometry to present a dual-pulse method using the thymidine analog 5-ethyl-2'-deoxyuridine (EdU), detected by click chemistry, combined with BrdU labeling and detection.

Sat Jan 01 00:00:00 EST 2011

Bioinformatics

Protein Aliases: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine

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BrdU Monoclonal Antibody (MoBU-1), Alexa Fluor™ 488

BrdU Antibody (B35139) in Flow

Product Details

B35139

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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