Invitrogen

Actin Polyclonal Antibody

1 Reference
View all (22) Actin antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-11571

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50
-
Product Specifications

Species Reactivity

Human

Published species

Plant

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 189-218 amino acids from the central region of human Actin (ACTB/ACTC)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Ammonium sulfate precipitation, Size-exclusion - Dialysis

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2223355

Product Specific Information

This antibody is predicted to react with bovine, chicken, canine, equine, hamster, mouse, porcine, non-human primate, rat, rabbit and Xenopus, yeast and zebrafish based on sequence homology.

Target Information

Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; Actin, aortic smooth muscle; actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 2; Actin, gamma-enteric smooth muscle; actin-like protein; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; Atcb; beta cytoskeletal actin; Beta-actin; Cell growth-inhibiting gene 46 protein; cytoskeletal gamma-actin; deafness, autosomal dominant 20; deafness, autosomal dominant 26; epididymis luminal protein 176; Gamma-2-actin; Gamma-actin; nemaline myopathy type 3; pan actin; PS1TP5-binding protein 1; RP5-1068B5.2; Smooth muscle gamma-actin

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Gene Aliases: AAT6; ACT; ACTA; ACTA1; ACTA2; ACTA3; ACTB; ACTC; ACTC1; ACTE; ACTG; ACTG1; ACTG2; ACTL3; ACTSA; ACTSG; ACTVS; ASD5; ASMA; BRWS1; BRWS2; CFTD; CFTD1; CFTDM; CMD1R; CMH11; DFNA20; DFNA26; GIG46; HEL-176; LVNC4; MPFD; MYMY5; NEM1; NEM2; NEM3; PS1TP5BP1; SHPM; VSCM

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UniProt ID: (Human) P68133, (Human) P62736, (Human) P60709, (Human) P68032, (Human) P63261, (Human) P63267

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Entrez Gene ID: (Human) 58, (Human) 59, (Human) 60, (Human) 70, (Human) 71, (Human) 72

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Function(s)
structural constituent of cytoskeleton protein binding ATP binding myosin binding ADP binding protein kinase binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding kinesin binding Tat protein binding nucleosomal DNA binding identical protein binding nitric-oxide synthase binding ATPase activity ubiquitin protein ligase binding actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein
Process(es)
muscle contraction response to mechanical stimulus response to extracellular stimulus response to lithium ion positive regulation of gene expression cell growth muscle filament sliding skeletal muscle thin filament assembly skeletal muscle fiber adaptation response to steroid hormone skeletal muscle fiber development mesenchyme migration regulation of blood pressure response to virus vascular smooth muscle contraction glomerular mesangial cell development retina homeostasis movement of cell or subcellular component cytoskeleton organization substantia nigra development cell junction assembly Fc-gamma receptor signaling pathway involved in phagocytosis ATP-dependent chromatin remodeling positive regulation of gene expression, epigenetic vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway platelet aggregation apoptotic process actin filament-based movement actomyosin structure organization actin-myosin filament sliding response to drug negative regulation of apoptotic process response to ethanol cardiac myofibril assembly cardiac muscle tissue morphogenesis heart contraction cardiac muscle contraction sarcomere organization
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