Invitrogen

ALDH2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Independent antibody to ensure that the antibody binds to the antigen stated.

1 Published Figure

1 Reference

View all (26) ALDH2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

ALDH2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

ALDH2 Antibody (PA5-11483) in ICC/IF

Immunofluorescence analysis of ALDH2 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ALDH2 Polyclonal Antibody (Product # PA5-11483) at 1:50 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a... View More

Product Image Gallery

ALDH2 Antibody — Antibody specificity was demonstrated by showing that antibodies raised against the same target protein perform similarly. Immunofluorescence of ALDH2 using ALDH2 Polyclonal Antibody (Product # PA5-11483) performed along with another ALDH2 antibody (Product # PA5-29717) shows similar localization pattern in Hep G2 cells. {IAV}

Product Details

PA5-11483

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:50

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 52-81 amino acids from the N-terminal region of human ALDH2

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.0 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2224191

Target Information

ALDH2 belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Asians have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Asians than among Caucasians could be related to the absence of the mitochondrial isozyme.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Critical role of mitochondrial aldehyde dehydrogenase 2 in acrolein sequestering in rat spinal cord injury.

Neural regeneration research

Herr SA,Shi L,Gianaris T,Jiao Y,Sun S,Race N,Shapiro S,Shi R

Published figure using ALDH2 polyclonal antibody (Product # PA5-11483) in Western Blot

Fri Jul 01 00:00:00 EDT 2022

Bioinformatics

Protein Aliases: acetaldehyde dehydrogenase 2; AHD-M1; aldehyde dehydrogenase 2, mitochondrial; Aldehyde dehydrogenase, mitochondrial; ALDH class 2; ALDH-E2; ALDH1; ALDHI; EC 1.2.1.3; liver mitochondrial ALDH; MGC1806; mitochondrial aldehyde dehydrogenase; nucleus-encoded mitochondrial aldehyde dehydrogenase 2

View more View less

Gene Aliases: Ahd-1; Ahd-5; AHD-M1; Ahd1; Ahd5; ALDH-E2; ALDH2; ALDHI; ALDM

View more View less

UniProt ID: (Human) P05091, (Rat) P11884, (Mouse) P47738

View more View less

Entrez Gene ID: (Human) 217, (Rat) 29539, (Mouse) 11669

View more View less

Function(s)

aldehyde dehydrogenase (NAD) activity aldehyde dehydrogenase [NAD(P)+] activity electron carrier activity protein binding oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor identical protein binding NADH binding dehydrogenase oxidoreductase metabolite interconversion enzyme

Process(es)

carbohydrate metabolic process alcohol metabolic process ethanol catabolic process ethanol oxidation liver development response to estradiol response to lipopolysaccharide response to progesterone cellular response to hormone stimulus response to testosterone response to nicotine negative regulation of apoptotic process response to hyperoxia oxidation-reduction process cellular response to fatty acid

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

ALDH2 Polyclonal Antibody

ALDH2 Antibody (PA5-11483) in ICC/IF

Product Details

PA5-11483

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!