Invitrogen

ACOX1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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Datasheet

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ACOX1 Antibody (PA5-29124) in WB

Western blot was performed using Anti-ACOX1 Polyclonal Antibody (Product # PA5-29124) and 72 kDa, 51 kDa bands corresponding to ACOX1 was observed in tissues tested except in Mouse Cerebellum, which is reported negative for ACOX1 expression for both isoforms and the 51 kDa isoform in Mouse Lung, a low expressing model for the same. Tissue extracts (30 µg lysate) of Mouse Lung (Lane 1), Mouse Cerebellum (Lane 2), Mouse Kidney (Lane 3), Mouse Liver (Lane 4) and Rat Liver (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (P... View More

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ACOX1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues tested owing to their inherent genetic constitution. Relative expression of ACOX1 was observed in higher levels in Mouse Liver in comparison to Mouse Lung and Mouse Cerebellum for both the isoforms using Anti-ACOX1 Polyclonal Antibody (Product # PA5-29124) in Western Blot. (doi:10.1016/j.bbadis.2017.12.032). {RE}

Product Details

PA5-29124

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 216 and 481 of Human ACOX1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.89 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2546600

Product Specific Information

Recommended positive controls: HepG2.

Predicted reactivity: Mouse (88%), Rat (89%), Pig (91%), Chicken (82%), Rhesus Monkey (100%), Bovine (92%), Guinea pig (89%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It donates electrons directly to molecular oxygen, thereby producing hydrogen peroxide. Defects in this gene result in pseudoneonatal adrenoleukodystrophy, a disease that is characterized by accumulation of very long chain fatty acids. Alternatively spliced transcript variants encoding different isoforms have been identified.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ACO; acyl-CoA oxidase 1, palmitoyl; acyl-CoA oxidase, straight-chain; acyl-Coenzyme A oxidase 1, palmitoyl; AOX; Palmitoyl-CoA oxidase; Peroxisomal acyl-coenzyme A oxidase 1; Peroxisomal fatty acyl-CoA oxidase; peroxisomal fatty acyl-coenzyme A oxidase; peroxisomal straight-chain Acyl-CoA oxidase; SCOX; Straight-chain acyl-CoA oxidase

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Gene Aliases: ACOX; ACOX1; AOX; D130055E20Rik; PALMCOX; Paox; RATACOA1; SCOX

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UniProt ID: (Human) Q15067, (Rat) P07872, (Mouse) Q9R0H0

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Entrez Gene ID: (Human) 51, (Rat) 50681, (Mouse) 11430

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Function(s)

fatty-acyl-CoA binding acyl-CoA dehydrogenase activity acyl-CoA oxidase activity receptor binding electron carrier activity PDZ domain binding protein N-terminus binding flavin adenine dinucleotide binding oxidoreductase activity, acting on the CH-CH group of donors, with a flavin as acceptor fatty acid binding palmitoyl-CoA oxidase activity FAD binding oxidoreductase activity oxidoreductase activity, acting on the CH-CH group of donors oxidoreductase

Process(es)

very long-chain fatty acid metabolic process generation of precursor metabolites and energy lipid metabolic process prostaglandin metabolic process spermatogenesis fatty acid oxidation fatty acid beta-oxidation using acyl-CoA dehydrogenase fatty acid beta-oxidation using acyl-CoA oxidase alpha-linolenic acid metabolic process fatty acid beta-oxidation peroxisome fission lipid homeostasis positive regulation of cholesterol homeostasis fatty acid metabolic process metabolic process oxidation-reduction process

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ACOX1 Polyclonal Antibody

ACOX1 Antibody (PA5-29124) in WB

Product Details

PA5-29124

Western Blot (WB)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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