Invitrogen

ACE2 Polyclonal Antibody

ACE2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

ACE2 Antibody (PA5-20046) in ICC/IF

Immunofluorescent analysis of 4% paraformaldehyde-fixed Caco2 cells labeling ACE2 with ACE2 Polyclonal Antibody (Product # PA5-20046) at 5 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI staining (blue). Image showing membrane staining on Caco2 cells.

ACE2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ACE2 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

ACE2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

PA5-20046

Applications

Tested Dilution

Publications

Western Blot (WB)

4 µg/mL

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

2 µg/mL

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

20 µg/mL

Immunocytochemistry (ICC/IF)

20 µg/mL

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Non-human primate

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to amino acids near the center of human ACE2.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11152624

Product Specific Information

In Western blot applications, this antibody detects a band at ~90kDa. A suggested positive control is human kidney tissue lysate.

PA5-20046 can be used with blocking peptide PEP-0165.

Target Information

Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Human lungs show limited permissiveness for SARS-CoV-2 due to scarce ACE2 levels but virus-induced expansion of inflammatory macrophages.

The European respiratory journal

Hönzke K,Obermayer B,Mache C,Fatykhova D,Kessler M,Dökel S,Wyler E,Baumgardt M,Löwa A,Hoffmann K,Graff P,Schulze J,Mieth M,Hellwig K,Demir Z,Biere B,Brunotte L,Mecate-Zambrano A,Bushe J,Dohmen M,Hinze C,Elezkurtaj S,Tönnies M,Bauer TT,Eggeling S,Tran HL,Schneider P,Neudecker J,Rückert JC,Schmidt-Ott KM,Busch J,Klauschen F,Horst D,Radbruch H,Radke J,Heppner F,Corman VM,Niemeyer D,Müller MA,Goffinet C,Mothes R,Pascual-Reguant A,Hauser AE,Beule D,Landthaler M,Ludwig S,Suttorp N,Witzenrath M,Gruber AD,Drosten C,Sander LE,Wolff T,Hippenstiel S,Hocke AC

PA5-20046 was used in Immunohistochemistry-immunofluorescence to indicate that severe lung injury in COVID-19 probably results from a macrophage-triggered immune activation rather than direct viral damage of the alveolar compartment.

Thu Dec 01 00:00:00 EST 2022

Tunneling nanotubes provide a route for SARS-CoV-2 spreading.

Science advances

Pepe A,Pietropaoli S,Vos M,Barba-Spaeth G,Zurzolo C

PA5-20046 was used in Immunocytochemistry-immunofluorescence to highlight a previously unknown mechanism of SARS-CoV-2 spreading, likely used as a route to invade nonpermissive cells and potentiate infection in permissive cells.

Fri Jul 22 00:00:00 EDT 2022

Tunneling nanotubes provide a route for SARS-CoV-2 spreading.

Science advances

Pepe A,Pietropaoli S,Vos M,Barba-Spaeth G,Zurzolo C

Fri Jul 22 00:00:00 EDT 2022

Paradoxical effects of cigarette smoke and COPD on SARS-CoV-2 infection and disease.

BMC pulmonary medicine

Tomchaney M,Contoli M,Mayo J,Baraldo S,Li S,Cabel CR,Bull DA,Lick S,Malo J,Knoper S,Kim SS,Tram J,Rojas-Quintero J,Kraft M,Ledford JG,Tesfaigzi Y,Martinez FD,Thorne CA,Kheradmand F,Campos SK,Papi A,Polverino F

PA5-20046 was used immunohistochemistry (paraffin) investigate the effects of chronic obstructive pulmonary disease (COPD) and cigarette smoke (CS) on the expression of SARS-CoV-2 entry receptor ACE2 in vivo in COPD patients and controls and in CS-exposed mice, and the effects of CS on SARS-CoV-2 infection in human bronchial epithelial cells in vitro.

Mon Aug 23 00:00:00 EDT 2021

Paradoxical effects of cigarette smoke and COPD on SARS-CoV-2 infection and disease.

BMC pulmonary medicine

Mon Aug 23 00:00:00 EDT 2021

Differential Effect of SARS-CoV-2 Spike Glycoprotein 1 on Human Bronchial and Alveolar Lung Mucosa Models: Implications for Pathogenicity.

Viruses

Rahman M,Irmler M,Keshavan S,Introna M,Beckers J,Palmberg L,Johanson G,Ganguly K,Upadhyay S

PA5-20046 was used in Immunocytochemistry to observe a typical anti-viral response in the bronchial model and a pro-fibrotic response in the alveolar model.

Fri Dec 17 00:00:00 EST 2021

Bioinformatics

Protein Aliases: ACE 2; ACE-related carboxypeptidase; ACEH; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; Angiotensin-converting enzyme 2; Angiotensin-converting enzyme homolog; Angiotensin-converting enzyme-related carboxypeptidase; Metalloprotease MPROT15; peptidyl-dipeptidase A

View more View less

Gene Aliases: ACE2; ACEH; UNQ868/PRO1885

View more View less

UniProt ID: (Human) Q9BYF1

View more View less

Entrez Gene ID: (Human) 59272

View more View less

Function(s)

virus receptor activity glycoprotein binding endopeptidase activity carboxypeptidase activity metallocarboxypeptidase activity protein binding metallopeptidase activity peptidyl-dipeptidase activity zinc ion binding metalloprotease

Process(es)

regulation of cytokine production angiotensin maturation angiotensin catabolic process in blood angiotensin-mediated drinking behavior regulation of systemic arterial blood pressure by renin-angiotensin tryptophan transport regulation of vasoconstriction receptor biosynthetic process regulation of cell proliferation regulation of vasodilation viral entry into host cell receptor-mediated virion attachment to host cell regulation of inflammatory response positive regulation of cardiac muscle contraction positive regulation of gap junction assembly regulation of cardiac conduction positive regulation of reactive oxygen species metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

ACE2 Polyclonal Antibody

ACE2 Antibody (PA5-20046) in ICC/IF

Product Details

PA5-20046

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!