Invitrogen

SOD1 Recombinant Superclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 Published Figures

2 References

View all (78) SOD1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

SOD1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

SOD1 Antibody (711818) in ICC/IF

Immunofluorescence of SOD1 was performed using HeLa wild-type and SOD1 KO cells that were transfected with a green or a far red fluorescent dye, respectively. Post-transfection, WT and KO cells were mixed and plated to a 1:1 ratio on coverslips as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS with 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min. Cells were stained with the SOD1 recombinant polyclonal antibody ... View More

SOD1 Antibody in Immunoprecipitation (IP)

SOD1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

SOD1 Antibody — Antibody specificity was demonstrated by siRNA-mediated knockdown of target protein. HeLa cells were transfected with SOD1 specific siRNA and decrease in signal intensity was observed in immunofluorescence application using Anti-SOD1 Recombinant Rabbit Polyclonal Antibody (Product # 711818). {KD}

Product Details

711818

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Immunogen

Protein corresponding to human SOD1 (aa1-aa154)

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2688303

Product Specific Information

This antibody is predicted to react with Monkey, Mouse, and Rat

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

Target Information

SOD1 (superoxide dismutase) binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Identification of high-performing antibodies for Superoxide dismutase [Cu-Zn] 1 (SOD1) for use in Western blot, immunoprecipitation, and immunofluorescence.

F1000Research

Ayoubi R,Alshafie W,You Z,Southern K,McPherson PS,Laflamme C

Published figure using SOD1 recombinant polyclonal antibody (Product # 711818) in Western Blot

Mon Oct 23 00:00:00 EDT 2023

Identification of high-performing antibodies for Superoxide dismutase [Cu-Zn] 1 (SOD1) for use in Western blot, immunoprecipitation, and immunofluorescence.

F1000Research

Ayoubi R,Alshafie W,You Z,Southern K,McPherson PS,Laflamme C

Published figure using SOD1 recombinant polyclonal antibody (Product # 711818) in Western Blot

Mon Oct 23 00:00:00 EDT 2023

Bioinformatics

Protein Aliases: Amyotrophic lateral sclerosis 1 (ALS1); Cu/Zn SOD; Cu/Zn superoxide dismutase; epididymis secretory protein Li 44; HGNC:11179; hSod1; indophenoloxidase A; Indophenoloxidase A (IPOA); SOD, soluble; superoxide dismutase; Superoxide dismutase 1; Superoxide Dismutase 1 (SOD1); Superoxide dismutase [Cu-Zn]; superoxide dismutase, cystolic

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Gene Aliases: ALS; ALS1; HEL-S-44; homodimer; hSod1; IPOA; SOD; SOD1

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UniProt ID: (Human) P00441

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Entrez Gene ID: (Human) 6647

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Function(s)

superoxide dismutase activity copper ion binding protein binding zinc ion binding protein phosphatase 2B binding identical protein binding protein homodimerization activity Rac GTPase binding chaperone binding oxidoreductase

Process(es)

activation of MAPK activity response to reactive oxygen species response to superoxide ovarian follicle development positive regulation of cytokine production placenta development retina homeostasis response to amphetamine myeloid cell homeostasis platelet degranulation glutathione metabolic process superoxide metabolic process cellular iron ion homeostasis spermatogenesis embryo implantation cell aging sensory perception of sound locomotory behavior anterograde axonal transport retrograde axonal transport regulation of blood pressure response to heat response to organic substance transmission of nerve impulse removal of superoxide radicals response to nutrient levels peripheral nervous system myelin maintenance positive regulation of superoxide anion generation regulation of T cell differentiation in thymus response to carbon monoxide cellular response to potassium ion regulation of multicellular organism growth response to drug response to hydrogen peroxide superoxide anion generation positive regulation of apoptotic process positive regulation of catalytic activity regulation of GTPase activity negative regulation of neuron apoptotic process response to ethanol negative regulation of cholesterol biosynthetic process regulation of protein kinase activity regulation of organ growth response to antibiotic response to copper ion muscle cell cellular homeostasis thymus development response to axon injury hydrogen peroxide biosynthetic process regulation of mitochondrial membrane potential oxidation-reduction process heart contraction neurofilament cytoskeleton organization relaxation of vascular smooth muscle auditory receptor cell stereocilium organization cellular response to cadmium ion cellular response to ATP reactive oxygen species metabolic process response to antipsychotic drug positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SOD1 Recombinant Superclonal Antibody

SOD1 Antibody (711818) in ICC/IF

Product Details

711818

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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