Invitrogen

6x-His Tag Monoclonal Antibody (4E3D10H2/E3), Alexa Fluor™ 647

6x-His Tag Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 3

6x-His Tag Antibody (MA1-135-A647) in ICC/IF

Immunofluorescent analysis of HeLa cells transfected with a construct containing a HIS Epitope Tag. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 inPBS for 15 minutes at room temperature and blocked with 3% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were stained with AlexaFluor 647 conjugated HIS-tag monoclonal antibody (Product # MA1-135-A647) ... View More

6x-His Tag Antibody in Western Blot (WB)

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Product Details

MA1-135-A647

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunocytochemistry (ICC/IF)

1:25-1:100

Flow Cytometry (Flow)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4E3D10H2/E3

Immunogen

Mixture of N-terminal and C-terminal 6x-His peptides, CG-HHHHHH and HHHHHH-GC

Conjugate

Alexa Fluor™ 647 Alexa Fluor™ 647 Alexa Fluor™ 647

Excitation/Emission Max

650/671 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2610637

Target Information

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Genetically encoded chemical crosslinking of carbohydrate.

Nature chemistry

Li S,Wang N,Yu B,Sun W,Wang L

MA1-135-A647 was used in Flow Cytometry to show that sulfonyl fluoride reacts with glycans via a proximity-enabled reactivity, and to harness this a bioreactive unnatural amino acid (SFY) that contains sulfonyl fluoride was genetically encoded into proteins.

Sun Jan 01 00:00:00 EST 2023

SynNotch CAR circuits enhance solid tumor recognition and promote persistent antitumor activity in mouse models.

Science translational medicine

Hyrenius-Wittsten A,Su Y,Park M,Garcia JM,Alavi J,Perry N,Montgomery G,Liu B,Roybal KT

MA1-135-A647 was used in Flow cytometry/Cell sorting to evaluate the effectiveness of synNotch CAR circuits as a tumor suppressor in mouse models.

Wed Apr 28 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: 6His tag; 6X His; His Tag; Histidine Tag

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