Invitrogen

Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP

Mouse IgG (H+L) Secondary Antibody in Western Blot (WB)

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Mouse IgG (H+L) Secondary Antibody (62-6520) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of T47D (Lane 1), A549 (Lane 2), U2OS (Lane 3), K562 (Lane 4), HeLa (Lane 5), Raji (lane 6), HEK-MSR (lane 7), MCF7 (lane 8), MDA-MB-231 (lane 9), LNCaP (lane 10) and Hep G2 (lane 11). The blots were probed with Anti-PRMT3 Mouse Monoclonal Antibody (Product # 730020, 0.5-1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse... View More

Mouse IgG (H+L) Secondary Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Mouse IgG (H+L) Secondary Antibody in Western Blot, Immunoprecipitation (WB, IP)

Mouse IgG (H+L) Secondary Antibody in ELISA (ELISA)

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Product Details

62-6520

Applications

Tested Dilution

Western Blot (WB)

1:2,000-1:10,000

Immunohistochemistry (IHC)

1:2,000-1:4,000

ELISA (ELISA)

1:2,000-1:4,000

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

HRP HRP HRP

Form

Liquid

Concentration

1.5 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4, with 4mg/mL BSA, 40% glycerol

Contains

0.19% Kathon™ CG

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533947

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

ZyMAX antibodies are specifically isolated from antigen-affinity columns using advanced elution protocols, leaving only the highest affinity, antigen-specific antibodies. ZyMAX conjugates are prepared with modified cross-linkers to achieve optimal conjugation ratios and stability. Improved purification methods virtually eliminate unconjugated components, giving superior sensitivity and lowest possible levels of background.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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