Invitrogen

CD123 Monoclonal Antibody (6H6), eFluor™ 450, eBioscience™

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CD123 Antibody (48-1239-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD85g (ILT7) PE (Product # 12-5179-42) and Mouse IgG1 kappa Isotype Control eFluor® 450 (Product # 48-4714-82) (left) or Anti-Human CD123 eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.

CD123 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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Product Details

48-1239-42

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 13 publications 13 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

6H6

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1548710

Product Specific Information

Description: The 6H6 monoclonal antibody reacts with human CD123, the alpha chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself, and when associated with CD131 (common beta chain) binds IL-3 with high affinity. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes.

Applications Reported: This 6H6 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 6H6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-3Ra (Interleukin-3 receptor subunit alpha) belongs to the type I cytokine receptor family and is a heterodimer with a unique alpha chain paired with the common beta (beta c or CDw131) subunit. IL-3Ra is responsible for the transmission of signal of Interleukin-3. IL-3Ra and IL-3Rb chain heterodimerization is induced by Interleukin-3, which is required for receptor activation but not high affinity binding. IL-3Ra is also reported that IL-3Ra subunits are overexpressed on acute myeloid leukemia (AML) blasts compared with normal hematopoietic cells and are thus potential targets for novel therapeutic agents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Unsupervised Analysis of Flow Cytometry Data in a Clinical Setting Captures Cell Diversity and Allows Population Discovery.

Frontiers in immunology

Baumgaertner P,Sankar M,Herrera F,Benedetti F,Barras D,Thierry AC,Dangaj D,Kandalaft LE,Coukos G,Xenarios I,Guex N,Harari A

Published figure using CD123 monoclonal antibody (Product # 48-1239-42) in Flow Cytometry

Tue Sep 21 00:00:00 EDT 2021

Single-Cell Analyses Reveal Megakaryocyte-Biased Hematopoiesis in Myelofibrosis and Identify Mutant Clone-Specific Targets.

Molecular cell

Psaila B,Wang G,Rodriguez-Meira A,Li R,Heuston EF,Murphy L,Yee D,Hitchcock IS,Sousos N,O'Sullivan J,Anderson S,Senis YA,Weinberg OK,Calicchio ML,Iskander D,Royston D,Milojkovic D,Roberts I,Bodine DM,Thongjuea S,Mead AJ

Published figure using CD123 monoclonal antibody (Product # 48-1239-42) in Flow Cytometry

Thu May 07 00:00:00 EDT 2020

Systems Biology Methods Applied to Blood and Tissue for a Comprehensive Analysis of Immune Response to Hepatitis B Vaccine in Adults.

Frontiers in immunology

Ben-Othman R,Cai B,Liu AC,Varankovich N,He D,Blimkie TM,Lee AH,Gill EE,Novotny M,Aevermann B,Drissler S,Shannon CP,McCann S,Marty K,Bjornson G,Edgar RD,Lin DTS,Gladish N,Maclsaac J,Amenyogbe N,Chan Q,Llibre A,Collin J,Landais E,Le K,Reiss SM,Koff WC,Havenar-Daughton C,Heran M,Sangha B,Walt D,Krajden M,Crotty S,Sok D,Briney B,Burton DR,Duffy D,Foster LJ,Mohn WW,Kobor MS,Tebbutt SJ,Brinkman RR,Scheuermann RH,Hancock REW,Kollmann TR,Sadarangani M

Published figure using CD123 monoclonal antibody (Product # 48-1239-42) in Flow Cytometry

Mon Jun 21 00:00:00 EDT 2021

Neutrophils Inhibit Synthesis of Mineralized Extracellular Matrix by Human Bone Marrow-Derived Stromal Cells In Vitro.

Frontiers in immunology

Bastian OW,Croes M,Alblas J,Koenderman L,Leenen LPH,Blokhuis TJ

Published figure using CD123 monoclonal antibody (Product # 48-1239-42) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2019

IL-15 Promotes Polyfunctional NK Cell Responses to Influenza by Boosting IL-12 Production.

Journal of immunology (Baltimore, Md. : 1950)

Wagstaffe HR,Nielsen CM,Riley EM,Goodier MR

48-1239 was used in Flow cytometry/Cell sorting to suggest that very low concentrations of IL-15 play an important role in boosting accessory cell function to support NK cell effector functions.

Sun Apr 15 00:00:00 EDT 2018

Enhancement of cytokine-driven NK cell IFN-γ production after vaccination of HCMV infected Africans.

European journal of immunology

Darboe A,Danso E,Clarke E,Umesi A,Touray E,Wegmuller R,Moore SE,Riley EM,Goodier MR

48-1239-42 was used in Flow Cytometry to investigate the impact of advanced NK cell differentiation on vaccine-induced responses.

Thu Jun 01 00:00:00 EDT 2017

Communication between Human Dendritic Cell Subsets in Tuberculosis: Requirements for Naive CD4(+) T Cell Stimulation.

Frontiers in immunology

Lozza L,Farinacci M,Bechtle M,Stäber M,Zedler U,Baiocchini A,Del Nonno F,Kaufmann SH

48-1239 was used in Flow cytometry/Cell sorting to study the response of different primary dendritic cell subsets to Mycobacterium tuberculosis infection.

Tue Jul 29 00:00:00 EDT 2014

Malaria-induced NLRP12/NLRP3-dependent caspase-1 activation mediates inflammation and hypersensitivity to bacterial superinfection.

PLoS pathogens

Ataide MA,Andrade WA,Zamboni DS,Wang D,Souza Mdo C,Franklin BS,Elian S,Martins FS,Pereira D,Reed G,Fitzgerald KA,Golenbock DT,Gazzinelli RT

Published figure using CD123 monoclonal antibody (Product # 48-1239-42) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

Crosstalk between human DC subsets promotes antibacterial activity and CD8+ T-cell stimulation in response to bacille Calmette-Guérin.

European journal of immunology

Lozza L,Farinacci M,Faé K,Bechtle M,Stäber M,Dorhoi A,Bauer M,Ganoza C,Weber S,Kaufmann SH

48-1239 was used in Flow cytometry/Cell sorting to study the role of human plasmacytoid DCs and myeloid DCs in the immune response to the tuberculosis vaccine bacille Calmette-Guérin.

Wed Jan 01 00:00:00 EST 2014

Frailty in old age is associated with decreased interleukin-12/23 production in response to toll-like receptor ligation.

PloS one

Compté N,Zouaoui Boudjeltia K,Vanhaeverbeek M,De Breucker S,Tassignon J,Trelcat A,Pepersack T,Goriely S

Published figure using CD123 monoclonal antibody (Product # 48-1239-42) in Flow Cytometry

Tue Jan 14 00:00:00 EST 2014

TNF receptor 1 genetic risk mirrors outcome of anti-TNF therapy in multiple sclerosis.

Nature

Gregory AP,Dendrou CA,Attfield KE,Haghikia A,Xifara DK,Butter F,Poschmann G,Kaur G,Lambert L,Leach OA,Prömel S,Punwani D,Felce JH,Davis SJ,Gold R,Nielsen FC,Siegel RM,Mann M,Bell JI,McVean G,Fugger L

48-1239 was used in Immunofluorescence to elucidate the causal SNP variant in the TNFRSF1A gene that is associated with multiple sclerosis, showing that rs1800693 is the causal variant, and that this expresses a soluble form of TNFR1 that can block TNF.

Thu Aug 23 00:00:00 EDT 2012

Multiplexed immunophenotyping of human antigen-presenting cells in whole blood by polychromatic flow cytometry.

Nature protocols

Fung E,Esposito L,Todd JA,Wicker LS

48-1239 was used in Flow cytometry/Cell sorting to develop two modular protocols for immunostaining and multiparameter flow cytometric analysis of major human antigen-presenting cells in minimally manipulated whole blood samples.

Mon Feb 01 00:00:00 EST 2010

Irreversible loss of pDCs by apoptosis during early HIV infection may be a critical determinant of immune dysfunction.

Viral immunology

Meera S,Madhuri T,Manisha G,Ramesh P

Published figure using CD123 monoclonal antibody (Product # 48-1239-42) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2010

Epithelial cells trigger frontline immunoglobulin class switching through a pathway regulated by the inhibitor SLPI.

Nature immunology

Xu W,He B,Chiu A,Chadburn A,Shan M,Buldys M,Ding A,Knowles DM,Santini PA,Cerutti A

Thu Mar 01 00:00:00 EST 2007

Local activation of the innate immune system in Buruli ulcer lesions.

The Journal of investigative dermatology

Peduzzi E,Groeper C,Schütte D,Zajac P,Rondini S,Mensah-Quainoo E,Spagnoli GC,Pluschke G,Daubenberger CA

Thu Mar 01 00:00:00 EST 2007

CD56-positive haematological neoplasms of the skin: a multicentre study of the Cutaneous Lymphoma Project Group of the European Organisation for Research and Treatment of Cancer.

Journal of clinical pathology

Assaf C,Gellrich S,Whittaker S,Robson A,Cerroni L,Massone C,Kerl H,Rose C,Chott A,Chimenti S,Hallermann C,Petrella T,Wechsler J,Bagot M,Hummel M,Bullani-Kerl K,Bekkenk MW,Kempf W,Meijer CJ,Willemze R,Sterry W

Published figure using CD123 monoclonal antibody (Product # 48-1239-42) in Immunohistochemistry

Sat Sep 01 00:00:00 EDT 2007

Expression of the plasmacytoid dendritic cell marker BDCA-2 supports a spectrum of maturation among CD4+ CD56+ hematodermic neoplasms.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Jaye DL,Geigerman CM,Herling M,Eastburn K,Waller EK,Jones D

Fri Dec 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: CD123; CD123 antigen; IL-3 receptor alpha SP2 isoform; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA; interleukin 3 receptor, alpha (low affinity); Interleukin-3 receptor subunit alpha

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Gene Aliases: CD123; hIL-3Ra; IL3R; IL3RA; IL3RAY; IL3RX; IL3RY

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UniProt ID: (Human) P26951

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Entrez Gene ID: (Human) 3563

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Function(s)

protein tyrosine kinase activity interleukin-3 receptor activity Ras guanyl-nucleotide exchange factor activity transmembrane signal receptor

Process(es)

MAPK cascade peptidyl-tyrosine phosphorylation cellular response to interleukin-3 interleukin-3-mediated signaling pathway positive regulation of GTPase activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD123 Monoclonal Antibody (6H6), eFluor™ 450, eBioscience™

CD123 Antibody (48-1239-42) in Flow

Product Details

48-1239-42

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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