Invitrogen

CD115 (c-fms) Monoclonal Antibody (AFS98), PerCP-eFluor™ 710, eBioscience™

CD115 (c-fms) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 27

CD115 (c-fms) Antibody (46-1152-82) in Flow

Staining of BALB/c thioglycolate-induced peritoneal exudate cells with 0.03 µg of Rat IgG2a K Isotype Control PerCP-eFluor® 710 (Product # 46-4321-82) (blue histogram) or 0.03 µg of Anti-Mouse CD115 (c-fms) PerCP-eFluor® 710 (purple histogram). Total viable cells were used for analysis.

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Product Details

46-1152-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.06 µg/test

View 30 publications 30 publications

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

AFS98

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10597740

Product Specific Information

Description: The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.

Applications Reported: This AFS98 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AFS98 antibody has been tested by flow cytometric analaysis of mouse thioglycolate induced peritoneal exudate cells. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CSF1R (FMS) is involved in the production, differentiation, and function of macrophages, and is implicated in promyelocytic leukemias. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. CSF1R is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in the gene encoding CSF1R have been associated with a predisposition to myeloid malignancy.Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and plays an important role in innate immunity and in inflammatory processes. The first intron of the CSF1R gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Mutations in the CSF1R gene have been associated with a predisposition to myeloid malignancy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Pyruvate dehydrogenase kinase supports macrophage NLRP3 inflammasome activation during acute inflammation.

Cell reports

Meyers AK,Wang Z,Han W,Zhao Q,Zabalawi M,Duan L,Liu J,Zhang Q,Manne RK,Lorenzo F,Quinn MA,Song Q,Fan D,Lin HK,Furdui CM,Locasale JW,McCall CE,Zhu X

Published figure using CD115 (c-fms) monoclonal antibody (Product # 46-1152-82) in Flow Cytometry

Tue Jan 31 00:00:00 EST 2023

Platelets exacerbate cardiovascular inflammation in a murine model of Kawasaki disease vasculitis.

JCI insight

Kocatürk B,Lee Y,Nosaka N,Abe M,Martinon D,Lane ME,Moreira D,Chen S,Fishbein MC,Porritt RA,Franklin BS,Noval Rivas M,Arditi M

Published figure using CD115 (c-fms) monoclonal antibody (Product # 46-1152-82) in Flow Cytometry

Mon Jul 24 00:00:00 EDT 2023

Pexidartinib synergize PD-1 antibody through inhibiting treg infiltration by reducing TAM-derived CCL22 in lung adenocarcinoma.

Frontiers in pharmacology

Zhang W,Jiang X,Zou Y,Yuan L,Wang X

Published figure using CD115 (c-fms) monoclonal antibody (Product # 46-1152-82) in Flow Cytometry

Tue Mar 28 00:00:00 EDT 2023

MARCH3 negatively regulates IL-3-triggered inflammatory response by mediating K48-linked polyubiquitination and degradation of IL-3Rα.

Signal transduction and targeted therapy

Feng L,Li C,Zeng LW,Gao D,Sun YH,Zhong L,Lin H,Shu HB,Li S

Published figure using CD115 (c-fms) monoclonal antibody (Product # 46-1152-82) in Flow Cytometry

Mon Jan 24 00:00:00 EST 2022

Loss of phosphatidylinositol-4-phosphate 5-kinase type-1 gamma (Pip5k1c) in mesenchymal stem cells leads to osteopenia by impairing bone remodeling.

The Journal of biological chemistry

Yan Q,Gao H,Yao Q,Ling K,Xiao G

Published figure using CD115 (c-fms) monoclonal antibody (Product # 46-1152-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2022

Gene-dosage effect of Pfkfb3 on monocyte/macrophage biology in atherosclerosis.

British journal of pharmacology

Guo S,Li A,Fu X,Li Z,Cao K,Song M,Huang S,Li Z,Yan J,Wang L,Dai X,Feng D,Wang Y,He J,Huo Y,Xu Y

46-1152-82 was used in Flow Cytometry to suggest the existence of a double-edged sword effect of myeloid PFKFB3 on the pathogenesis of atherosclerosis and highlight the need for caution in developing anti-atherosclerotic strategies that target PFKFB3.

Tue Nov 01 00:00:00 EDT 2022

Mapping the origin and fate of myeloid cells in distinct compartments of the eye by single-cell profiling.

The EMBO journal

Wieghofer P,Hagemeyer N,Sankowski R,Schlecht A,Staszewski O,Amann L,Gruber M,Koch J,Hausmann A,Zhang P,Boneva S,Masuda T,Hilgendorf I,Goldmann T,Böttcher C,Priller J,Rossi FM,Lange C,Prinz M

Published figure using CD115 (c-fms) monoclonal antibody (Product # 46-1152-82) in Flow Cytometry

Mon Mar 15 00:00:00 EDT 2021

Low-dose 2-deoxy glucose stabilises tolerogenic dendritic cells and generates potent in vivo immunosuppressive effects.

Cellular and molecular life sciences : CMLS

Christofi M,Le Sommer S,Mölzer C,Klaska IP,Kuffova L,Forrester JV

Published figure using CD115 (c-fms) monoclonal antibody (Product # 46-1152-82) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition).

European journal of immunology

Cossarizza A,Chang HD,Radbruch A,Abrignani S,Addo R,Akdis M,Andrä I,Andreata F,Annunziato F,Arranz E,Bacher P,Bari S,Barnaba V,Barros-Martins J,Baumjohann D,Beccaria CG,Bernardo D,Boardman DA,Borger J,Böttcher C,Brockmann L,Burns M,Busch DH,Cameron G,Cammarata I,Cassotta A,Chang Y,Chirdo FG,Christakou E,Čičin-Šain L,Cook L,Corbett AJ,Cornelis R,Cosmi L,Davey MS,De Biasi S,De Simone G,Del Zotto G,Delacher M,Di Rosa F,Di Santo J,Diefenbach A,Dong J,Dörner T,Dress RJ,Dutertre CA,Eckle SBG,Eede P,Evrard M,Falk CS,Feuerer M,Fillatreau S,Fiz-Lopez A,Follo M,Foulds GA,Fröbel J,Gagliani N,Galletti G,Gangaev A,Garbi N,Garrote JA,Geginat J,Gherardin NA,Gibellini L,Ginhoux F,Godfrey DI,Gruarin P,Haftmann C,Hansmann L,Harpur CM,Hayday AC,Heine G,Hernández DC,Herrmann M,Hoelsken O,Huang Q,Huber S,Huber JE,Huehn J,Hundemer M,Hwang WYK,Iannacone M,Ivison SM,Jäck HM,Jani PK,Keller B,Kessler N,Ketelaars S,Knop L,Knopf J,Koay HF,Kobow K,Kriegsmann K,Kristyanto H,Krueger A,Kuehne JF,Kunze-Schumacher H,Kvistborg P,Kwok I,Latorre D,Lenz D,Levings MK,Lino AC,Liotta F,Long HM,Lugli E,MacDonald KN,Maggi L,Maini MK,Mair F,Manta C,Manz RA,Mashreghi MF,Mazzoni A,McCluskey J,Mei HE,Melchers F,Melzer S,Mielenz D,Monin L,Moretta L,Multhoff G,Muñoz LE,Muñoz-Ruiz M,Muscate F,Natalini A,Neumann K,Ng LG,Niedobitek A,Niemz J,Almeida LN,Notarbartolo S,Ostendorf L,Pallett LJ,Patel AA,Percin GI,Peruzzi G,Pinti M,Pockley AG,Pracht K,Prinz I,Pujol-Autonell I,Pulvirenti N,Quatrini L,Quinn KM,Radbruch H,Rhys H,Rodrigo MB,Romagnani C,Saggau C,Sakaguchi S,Sallusto F,Sanderink L,Sandrock I,Schauer C,Scheffold A,Scherer HU,Schiemann M,Schildberg FA,Schober K,Schoen J,Schuh W,Schüler T,Schulz AR,Schulz S,Schulze J,Simonetti S,Singh J,Sitnik KM,Stark R,Starossom S,Stehle C,Szelinski F,Tan L,Tarnok A,Tornack J,Tree TIM,van Beek JJP,van de Veen W,van Gisbergen K,Vasco C,Verheyden NA,von Borstel A,Ward-Hartstonge KA,Warnatz K,Waskow C,Wiedemann A,Wilharm A,Wing J,Wirz O,Wittner J,Yang JHM,Yang J

46-1152-82 was used in Flow cytometry/Cell sorting to provide the key aspects to consider when performing flow cytometry experiments.

Wed Dec 01 00:00:00 EST 2021

HOXA11 plays critical roles in disease progression and response to cytarabine in AML.

Oncology reports

Fu JF,Shih LY,Yen TH

Published figure using CD115 (c-fms) monoclonal antibody (Product # 46-1152-82) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

Inhibition of TLR4 signaling protects mice from sensory and motor dysfunction in an animal model of autoimmune peripheral neuropathy.

Journal of neuroinflammation

Oladiran O,Shi XQ,Yang M,Fournier S,Zhang J

Published figure using CD115 (c-fms) monoclonal antibody (Product # 46-1152-82) in Flow Cytometry

Mon Mar 22 00:00:00 EDT 2021

CX3CR1 But Not CCR2 Expression Is Required for the Development of Autoimmune Peripheral Neuropathy in Mice.

Frontiers in immunology

Oladiran O,Shi XQ,Fournier S,Zhang J

Published figure using CD115 (c-fms) monoclonal antibody (Product # 46-1152-82) in Flow Cytometry

Tue Dec 21 00:00:00 EST 2021

Inflammation rapidly recruits mammalian GMP and MDP from bone marrow into regional lymphatics.

eLife

Serrano-Lopez J,Hegde S,Kumar S,Serrano J,Fang J,Wellendorf AM,Roche PA,Rangel Y,Carrington LJ,Geiger H,Grimes HL,Luther S,Maillard I,Sanchez-Garcia J,Starczynowski DT,Cancelas JA

46-1152-82 was used in Flow cytometry/Cell sorting to demonstrate that acute systemic inflammation induces the emergent activation of a previously unrecognized system of rapid migration of granulocyte-macrophage progenitors and committed macrophage-dendritic progenitors, but not other progenitors or stem cells, from bone marrow (BM) to regional lymphatic capillaries.

Thu Apr 08 00:00:00 EDT 2021

Inflammation rapidly recruits mammalian GMP and MDP from bone marrow into regional lymphatics.

eLife

Serrano-Lopez J,Hegde S,Kumar S,Serrano J,Fang J,Wellendorf AM,Roche PA,Rangel Y,Carrington LJ,Geiger H,Grimes HL,Luther S,Maillard I,Sanchez-Garcia J,Starczynowski DT,Cancelas JA

Thu Apr 08 00:00:00 EDT 2021

Hepatocyte-specific glucose-6-phosphatase deficiency disturbs platelet aggregation and decreases blood monocytes upon fasting-induced hypoglycemia.

Molecular metabolism

La Rose AM,Bazioti V,Hoogerland JA,Svendsen AF,Groenen AG,van Faassen M,Rutten MGS,Kloosterhuis NJ,Dethmers-Ausema B,Nijland JH,Mithieux G,Rajas F,Kuipers F,Lukens MV,Soehnlein O,Oosterveer MH,Westerterp M

Published figure using CD115 (c-fms) monoclonal antibody (Product # 46-1152-82) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

Radiation causes tissue damage by dysregulating inflammasome-gasdermin D signaling in both host and transplanted cells.

PLoS biology

Xiao J,Wang C,Yao JC,Alippe Y,Yang T,Kress D,Sun K,Kostecki KL,Monahan JB,Veis DJ,Abu-Amer Y,Link DC,Mbalaviele G

Published figure using CD115 (c-fms) monoclonal antibody (Product # 46-1152-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

Temporospatial distribution and transcriptional profile of retinal microglia in the oxygen-induced retinopathy mouse model.

Glia

Boeck M,Thien A,Wolf J,Hagemeyer N,Laich Y,Yusuf D,Backofen R,Zhang P,Boneva S,Stahl A,Hilgendorf I,Agostini H,Prinz M,Wieghofer P,Schlunck G,Schlecht A,Lange C

Published figure using CD115 (c-fms) monoclonal antibody (Product # 46-1152-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

Androgen Receptor Signaling Positively Regulates Monocytic Development.

Frontiers in immunology

Consiglio CR,Gollnick SO

Published figure using CD115 (c-fms) monoclonal antibody (Product # 46-1152-82) in Flow Cytometry

Thu Apr 29 00:00:00 EDT 2021

Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing the Liver Macrophage Niche.

Immunity

Bonnardel J,T'Jonck W,Gaublomme D,Browaeys R,Scott CL,Martens L,Vanneste B,De Prijck S,Nedospasov SA,Kremer A,Van Hamme E,Borghgraef P,Toussaint W,De Bleser P,Mannaerts I,Beschin A,van Grunsven LA,Lambrecht BN,Taghon T,Lippens S,Elewaut D,Saeys Y,Guilliams M

46-1152-82 was used in Flow Cytometry to show that the Kupffer cell niche is composed of stellate cells, hepatocytes, and endothelial cells that together imprint the liver-specific macrophage identity.

Tue Oct 15 00:00:00 EDT 2019

Concomitant type I IFN and M-CSF signaling reprograms monocyte differentiation and drives pro-tumoral arginase production.

EBioMedicine

Tong Y,Zhou L,Yang L,Guo P,Cao Y,Qin FX,Liu J

Published figure using CD115 (c-fms) monoclonal antibody (Product # 46-1152-82) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2019

Liver-Derived Signals Sequentially Reprogram Myeloid Enhancers to Initiate and Maintain Kupffer Cell Identity.

Immunity

Sakai M,Troutman TD,Seidman JS,Ouyang Z,Spann NJ,Abe Y,Ego KM,Bruni CM,Deng Z,Schlachetzki JCM,Nott A,Bennett H,Chang J,Vu BT,Pasillas MP,Link VM,Texari L,Heinz S,Thompson BM,McDonald JG,Geissmann F,Glass CK

46-1152-82 was used in Flow cytometry/Cell sorting to provide a framework for understanding how macrophage progenitor cells acquire tissue-specific phenotypes.

Tue Oct 15 00:00:00 EDT 2019

Bone loss in C57BL/6J-OlaHsd mice, a substrain of C57BL/6J carrying mutated alpha-synuclein and multimerin-1 genes.

Journal of cellular physiology

Liron T,Raphael B,Hiram-Bab S,Bab IA,Gabet Y

Published figure using CD115 (c-fms) monoclonal antibody (Product # 46-1152-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Captopril mitigates splenomegaly and myelofibrosis in the Gata1^low murine model of myelofibrosis.

Journal of cellular and molecular medicine

Corey SJ,Jha J,McCart EA,Rittase WB,George J,Mattapallil JJ,Mehta H,Ognoon M,Bylicky MA,Summers TA,Day RM

Published figure using CD115 (c-fms) monoclonal antibody (Product # 46-1152-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Neutrophil and Macrophage Cell Surface Colony-Stimulating Factor 1 Shed by ADAM17 Drives Mouse Macrophage Proliferation in Acute and Chronic Inflammation.

Molecular and cellular biology

Tang J,Frey JM,Wilson CL,Moncada-Pazos A,Levet C,Freeman M,Rosenfeld ME,Stanley ER,Raines EW,Bornfeldt KE

Published figure using CD115 (c-fms) monoclonal antibody (Product # 46-1152-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Advanced Glycation End Products Enhance Murine Monocyte Proliferation in Bone Marrow and Prime Them into an Inflammatory Phenotype through MAPK Signaling.

Journal of diabetes research

Jin X,Liu L,Zhang Y,Xiang Y,Yin G,Lu Y,Shi L,Dong J,Shen C

Published figure using CD115 (c-fms) monoclonal antibody (Product # 46-1152-82) in Flow Cytometry

Wed Oct 10 00:00:00 EDT 2018

Erythropoietin enhances Kupffer cell number and activity in the challenged liver.

Scientific reports

Gilboa D,Haim-Ohana Y,Deshet-Unger N,Ben-Califa N,Hiram-Bab S,Reuveni D,Zigmond E,Gassmann M,Gabet Y,Varol C,Neumann D

Published figure using CD115 (c-fms) monoclonal antibody (Product # 46-1152-82) in Flow Cytometry

Mon Sep 04 00:00:00 EDT 2017

Enforced Expression of Hoxa3 Inhibits Classical and Promotes Alternative Activation of Macrophages In Vitro and In Vivo.

Journal of immunology (Baltimore, Md. : 1950)

Al Sadoun H,Burgess M,Hentges KE,Mace KA

Published figure using CD115 (c-fms) monoclonal antibody (Product # 46-1152-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

PER1 prevents excessive innate immune response during endotoxin-induced liver injury through regulation of macrophage recruitment in mice.

Cell death & disease

Wang T,Wang Z,Yang P,Xia L,Zhou M,Wang S,Du J,Zhang J

Published figure using CD115 (c-fms) monoclonal antibody (Product # 46-1152-82) in Flow Cytometry

Thu Apr 07 00:00:00 EDT 2016

miR-126 and miR-126* repress recruitment of mesenchymal stem cells and inflammatory monocytes to inhibit breast cancer metastasis.

Nature cell biology

Zhang Y,Yang P,Sun T,Li D,Xu X,Rui Y,Li C,Chong M,Ibrahim T,Mercatali L,Amadori D,Lu X,Xie D,Li QJ,Wang XF

Published figure using CD115 (c-fms) monoclonal antibody (Product # 46-1152-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

In vivo inhibition of c-MYC in myeloid cells impairs tumor-associated macrophage maturation and pro-tumoral activities.

PloS one

Pello OM,Chèvre R,Laoui D,De Juan A,Lolo F,Andrés-Manzano MJ,Serrano M,Van Ginderachter JA,Andrés V

Published figure using CD115 (c-fms) monoclonal antibody (Product # 46-1152-82) in Flow Cytometry

Mon Feb 25 00:00:00 EST 2013

Phospholipase Cgamma2 mediates RANKL-stimulated lymph node organogenesis and osteoclastogenesis.

The Journal of biological chemistry

Chen Y,Wang X,Di L,Fu G,Chen Y,Bai L,Liu J,Feng X,McDonald JM,Michalek S,He Y,Yu M,Fu YX,Wen R,Wu H,Wang D

Published figure using CD115 (c-fms) monoclonal antibody (Product # 46-1152-82) in Flow Cytometry

Fri Oct 24 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: c-fms; CD115; CSF-1 receptor; CSF-1-R; CSFIR; EC 2.7.10.1; kinase CSFR; Macrophage colony-stimulating factor 1 receptor; OTTHUMP00000224093; Proto-oncogene c-Fms; proto-oncogene fms

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Gene Aliases: AI323359; CD115; CSF-1R; Csf1r; Csfmr; Fim-2; Fms; M-CSF-R; M-CSFR

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UniProt ID: (Mouse) P09581

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Entrez Gene ID: (Mouse) 12978

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Function(s)

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity macrophage colony-stimulating factor receptor activity protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein phosphatase binding cytokine binding protein homodimerization activity transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD115 (c-fms) Monoclonal Antibody (AFS98), PerCP-eFluor™ 710, eBioscience™

CD115 (c-fms) Antibody (46-1152-82) in Flow

Product Details

46-1152-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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