Invitrogen

CD1c Monoclonal Antibody (L161), PerCP-eFluor™ 710, eBioscience™

FIGURE: 1 / 6

CD1c Antibody (46-0015-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (left) or Anti-Human CD1c PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

46-0015-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 13 publications 13 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

L161

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10548936

Product Specific Information

Description: This L161 monoclonal antibody detects CD1c (also known as BDCA-1), a glycoprotein that is noncovalently linked to beta-2 microglobulin on thymocytes and antigen presenting cells such as dendritic and Langerhans cells. This molecule is also expressed on some circulating and marginal zone B cells, as well as in lymph nodes and germinal centers. CD1c is involved in the presentation of lipid antigens such as microbial fatty acids to effector T cells during the adaptive immune response. Finally, alternative splicing gives rise to three different isoforms of CD1c (soluble, membrane, and cytoplasmic/soluble isoforms).

Applications Reported: This L161 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This L161 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail, and requires vesicular acidification to bind lipid antigens.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Heme oxygenase-1 determines the cell fate of ferroptotic death of alveolar macrophages in COPD.

Frontiers in immunology

Li Y,Yang Y,Guo T,Weng C,Yang Y,Wang Z,Zhang L,Li W

Published figure using CD1c monoclonal antibody (Product # 46-0015-42) in Flow Cytometry

Wed May 24 00:00:00 EDT 2023

Engineered Human Dendritic Cell Exosomes as Effective Delivery System for Immune Modulation.

International journal of molecular sciences

Elsayed R,Elashiry M,Tran C,Yang T,Carroll A,Liu Y,Hamrick M,Cutler CW

Published figure using CD1c monoclonal antibody (Product # 46-0015-42) in Flow Cytometry

Tue Jul 11 00:00:00 EDT 2023

WT1 Pulsed Human CD141+ Dendritic Cell Vaccine Has High Potential in Solid Tumor-Targeted Immunotherapy.

International journal of molecular sciences

Cho SY,Jeong SM,Jeon YJ,Yang SJ,Hwang JE,Yoo BM,Kim HS

46-0015-42 was used in Flow cytometry/Cell sorting to develop a DC vaccine named CellgramDC-WT1 (CDW).

Thu Jan 12 00:00:00 EST 2023

Single-cell RNA-seq analysis reveals dual sensing of HIV-1 in blood Axl⁺ dendritic cells.

iScience

Brouiller F,Nadalin F,Bonté PE,Ait-Mohamed O,Delaugerre C,Lelièvre JD,Ginhoux F,Ruffin N,Benaroch P

46-0015-42 was used in flow cytometry to demonstrate that HIV-1 induced two main broad and intense transcriptional programs in different Axl+DCs potentially induced by different sensors; an NF-κB-mediated program that led to DC maturation and efficient CD4+ T cell activation, and a program mediated by STAT1/2 that activated type I IFN and ISG responses.

Fri Feb 17 00:00:00 EST 2023

GM-CSF-activated human dendritic cells promote type 1 T follicular helper cell polarization in a CD40-dependent manner.

Journal of cell science

Korniotis S,Saichi M,Trichot C,Hoffmann C,Amblard E,Viguier A,Grondin S,Noel F,Mattoo H,Soumelis V

46-0015-42 was used in Flow cytometry/Cell sorting to report that human blood CD1c+ dendritic cells activated by GM-CSF (also known as CSF2) drive the differentiation of naive CD4+ T cells into Tfh1 cells.

Tue Nov 01 00:00:00 EDT 2022

DNAM1 and TIGIT balance the T cell response, with low T cell TIGIT expression corresponding to inflammation in psoriatic disease.

Immunotherapy advances

Jacobs ME,Pouw JN,Olde Nordkamp MA,Radstake TRDJ,Leijten EFA,Boes M

Published figure using CD1c monoclonal antibody (Product # 46-0015-42) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Transcriptional and Functional Analysis of CD1c⁺ Human Dendritic Cells Identifies a CD163⁺ Subset Priming CD8⁺CD103⁺ T Cells.

Immunity

Bourdely P,Anselmi G,Vaivode K,Ramos RN,Missolo-Koussou Y,Hidalgo S,Tosselo J,Nuñez N,Richer W,Vincent-Salomon A,Saxena A,Wood K,Lladser A,Piaggio E,Helft J,Guermonprez P

46-0015-42 was used in Flow Cytometry to define DC3s as a lineage of inflammatory DCs endowed with a strong potential to regulate tumor immunity.

Tue Aug 18 00:00:00 EDT 2020

Transcriptional Basis of Mouse and Human Dendritic Cell Heterogeneity.

Cell

Brown CC,Gudjonson H,Pritykin Y,Deep D,Lavallée VP,Mendoza A,Fromme R,Mazutis L,Ariyan C,Leslie C,Pe'er D,Rudensky AY

46-0015 was used in Flow cytometry/Cell sorting to identify two principal cDC2 lineages defined by distinct developmental pathways and transcriptional regulators, including T-bet and RORγt, two key transcription factors known to define innate and adaptive lymphocyte subsets.

Thu Oct 31 00:00:00 EDT 2019

NR4A Expression by Human Marginal Zone B-Cells.

Antibodies (Basel, Switzerland)

Doyon-Laliberté K,Chagnon-Choquet J,Byrns M,Aranguren M,Memmi M,Chrobak P,Stagg J,Poudrier J,Roger M

46-0015-42 was used in Flow Cytometry to shed light on the Breg potential of MZ populations, and identify NR4A1-3 as potential Breg markers, which as for Tregs, may be involved in stabilization of a regulatory status.

Fri Oct 11 00:00:00 EDT 2019

Aryl Hydrocarbon Receptor Controls Monocyte Differentiation into Dendritic Cells versus Macrophages.

Immunity

Goudot C,Coillard A,Villani AC,Gueguen P,Cros A,Sarkizova S,Tang-Huau TL,Bohec M,Baulande S,Hacohen N,Amigorena S,Segura E

46-0015 was used in Flow cytometry/Cell sorting to elucidate the role of the aryl hydrocarbon receptor as a molecular switch for monocyte fate specification in response to micro-environmental factors.

Tue Sep 19 00:00:00 EDT 2017

Fluctuations in Blood Marginal Zone B-Cell Frequencies May Reflect Migratory Patterns Associated with HIV-1 Disease Progression Status.

PloS one

Gauvin J,Chagnon-Choquet J,Poudrier J,Roger M

46-0015 was used in Flow cytometry/Cell sorting to show that the immune response against HIV-1 may involve the recruitment of marginal zone-like B-cells to peripheral sites.

Mon Jul 10 00:00:00 EDT 2017

Malaria-induced NLRP12/NLRP3-dependent caspase-1 activation mediates inflammation and hypersensitivity to bacterial superinfection.

PLoS pathogens

Ataide MA,Andrade WA,Zamboni DS,Wang D,Souza Mdo C,Franklin BS,Elian S,Martins FS,Pereira D,Reed G,Fitzgerald KA,Golenbock DT,Gazzinelli RT

Published figure using CD1c monoclonal antibody (Product # 46-0015-42) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

The Journal of experimental medicine

Segura E,Durand M,Amigorena S

46-0015 was used in Flow cytometry/Cell sorting to demonstrate that freshly isolated lymphoid organ-resident human DCs, but not macrophages, display high intrinsic cross-presentation capacity.

Mon May 06 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: CD1c; CD1C antigen, c polypeptide; cortical thymocyte antigen CD1C; differentiation antigen CD1-alpha-3; RP11-101J8.3; T-cell surface glycoprotein CD1c

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Gene Aliases: BDCA1; CD1; CD1A; CD1C; R7

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UniProt ID: (Human) P29017

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Entrez Gene ID: (Human) 911

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Function(s)

beta-2-microglobulin binding endogenous lipid antigen binding exogenous lipid antigen binding glycolipid binding lipopeptide binding major histocompatibility complex protein

Process(es)

adaptive immune response T cell activation involved in immune response antigen processing and presentation, exogenous lipid antigen via MHC class Ib regulation of immune response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD1c Monoclonal Antibody (L161), PerCP-eFluor™ 710, eBioscience™

CD1c Antibody (46-0015-42) in Flow

Product Details

46-0015-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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