Invitrogen

TER-119 Monoclonal Antibody (TER-119), PerCP-Cyanine5.5, eBioscience™

TER-119 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 41

TER-119 Antibody (45-5921-82) in Flow

Staining of C57BL/6 bone marrow cells with Anti-Mouse CD45 APC (Product # 17-0451-82) and 0.25 µg of Rat IgG2b K Isotype Control PerCP-Cyanine5-5 (Product # 45-4031-80) (left) or 0.25 µg of Anti-Mouse TER-119 PerCP-Cyanine5-5 (right). Total viable cells were used for analysis.

Product Image Gallery

Product Details

45-5921-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 41 publications 41 publications

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PerCP-Cyanine5.5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TER-119

Conjugate

PerCP-Cyanine5.5 PerCP-Cyanine5.5 PerCP-Cyanine5.5

Excitation/Emission Max

489/679 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_925765

Product Specific Information

Description: The TER-119 monoclonal antibody reacts with mouse erythroid cells from early proerythroblast to mature erythrocyte stages. The TER-119 antigen is present in yolk sac, fetal and newborn liver, but is not expressed by cells carrying BFU-E and CFU-E activities. Several erythroleukemia cell lines tested so far are negative for expression of TER-119 antigen even after dimethylsulfoxide stimulation. Biochemical and molecular analysis of the TER-119 antigen indicate that this molecule is associated with the surface glycophorin A, but is not a typical glycophorin.

Applications Reported: This TER-119 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TER-119 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TER-119 is a lineage marker for erythroid cells from early proerythroblast to mature erythrocyte stages in adult blood, spleen, and bone marrow. It is also present in yolk sac, and fetal and newborn liver. The TER-119 antigen is not expressed by cells of earlier erythroid development at BFU-e (blast-forming unit erythroid) stage or CFU-e (colony-forming unit erythroid) stage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

The microbiota regulates hematopoietic stem cell fate decisions by controlling iron availability in bone marrow.

Cell stem cell

Zhang D,Gao X,Li H,Borger DK,Wei Q,Yang E,Xu C,Pinho S,Frenette PS

45-5921-82 was used in Flow cytometry/Cell sorting to reveal an intricate interplay between the microbiota, macrophages, and iron, and their essential roles in regulating critical HSC fate decisions under stress.

Thu Feb 03 00:00:00 EST 2022

An optimized protocol for the isolation of rare stromal cell populations from the mouse spleen.

STAR protocols

Alexandre YO,Mueller SN

45-5921-82 was used in Flow cytometry/Cell sorting to present an optimized protocol that describes the digestion and enrichment steps for the isolation and analysis of rare populations of stromal cells, including fibroblastic reticular cells, perivascular cells, and glial cells found in the spleen.

Fri Dec 16 00:00:00 EST 2022

Pannexin 1 channels facilitate communication between T cells to restrict the severity of airway inflammation.

Immunity

Medina CB,Chiu YH,Stremska ME,Lucas CD,Poon I,Tung KS,Elliott MR,Desai B,Lorenz UM,Bayliss DA,Ravichandran KS

45-5921-82 was used in Flow cytometry/Cell sorting to uncover an immunoregulatory role for the nucleotide release channel, Panx1, in T cell crosstalk during airway disease.

Tue Aug 10 00:00:00 EDT 2021

Engineered red blood cells carrying PCSK9 inhibitors persistently lower LDL and prevent obesity.

PloS one

Deshycka R,Sudaryo V,Huang NJ,Xie Y,Smeding LY,Choi MK,Ploegh HL,Lodish HF,Pishesha N

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Fri Dec 24 00:00:00 EST 2021

GRHL3 activates FSCN1 to relax cell-cell adhesions between migrating keratinocytes during wound reepithelialization.

JCI insight

Kashgari G,Venkatesh S,Refuerzo S,Pham B,Bayat A,Klein RH,Ramos R,Ta AP,Plikus MV,Wang PH,Andersen B

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Wed Sep 08 00:00:00 EDT 2021

The IRE1/XBP1 signaling axis promotes skeletal muscle regeneration through a cell non-autonomous mechanism.

eLife

Roy A,Tomaz da Silva M,Bhat R,Bohnert KR,Iwawaki T,Kumar A

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Tue Nov 23 00:00:00 EST 2021

SnRNA sequencing defines signaling by RBC-derived extracellular vesicles in the murine heart.

Life science alliance

Valkov N,Das A,Tucker NR,Li G,Salvador AM,Chaffin MD,Pereira De Oliveira Junior G,Kur I,Gokulnath P,Ziegler O,Yeri A,Lu S,Khamesra A,Xiao C,Rodosthenous R,Srinivasan S,Toxavidis V,Tigges J,Laurent LC,Momma S,Kitchen R,Ellinor P,Ghiran I,Das S

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Wed Dec 01 00:00:00 EST 2021

Hypoxia-sensing CAR T cells provide safety and efficacy in treating solid tumors.

Cell reports. Medicine

Kosti P,Opzoomer JW,Larios-Martinez KI,Henley-Smith R,Scudamore CL,Okesola M,Taher MYM,Davies DM,Muliaditan T,Larcombe-Young D,Woodman N,Gillett CE,Thavaraj S,Maher J,Arnold JN

45-5921-82 was used in Flow Cytometry to present a stringent hypoxia-sensing CAR T cell system that achieves selective expression of a pan-ErbB-targeted CAR within a solid tumor.

Tue Apr 20 00:00:00 EDT 2021

Hypoxia-sensing CAR T cells provide safety and efficacy in treating solid tumors.

Cell reports. Medicine

Kosti P,Opzoomer JW,Larios-Martinez KI,Henley-Smith R,Scudamore CL,Okesola M,Taher MYM,Davies DM,Muliaditan T,Larcombe-Young D,Woodman N,Gillett CE,Thavaraj S,Maher J,Arnold JN

45-5921-82 was used in Flow Cytometry to present a stringent hypoxia-sensing CAR T cell system that achieves selective expression of a pan-ErbB-targeted CAR within a solid tumor.

Tue Apr 20 00:00:00 EDT 2021

Sex Matters: Physiological Abundance of Immuno-Regulatory CD71+ Erythroid Cells Impair Immunity in Females.

Frontiers in immunology

Mashhouri S,Koleva P,Huynh M,Okoye I,Shahbaz S,Elahi S

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Mon Dec 20 00:00:00 EST 2021

Inhibition of TGFβ improves hematopoietic stem cell niche and ameliorates cancer-related anemia.

Stem cell research & therapy

Wang B,Wang Y,Chen H,Yao S,Lai X,Qiu Y,Cai J,Huang Y,Wei X,Guan Y,Wang T,Wang J,Xiang AP

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Mon Jan 18 00:00:00 EST 2021

Lef1 restricts ectopic crypt formation and tumor cell growth in intestinal adenomas.

Science advances

Heino S,Fang S,Lähde M,Högström J,Nassiri S,Campbell A,Flanagan D,Raven A,Hodder M,Nasreddin N,Xue HH,Delorenzi M,Leedham S,Petrova TV,Sansom O,Alitalo K

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Fri Nov 19 00:00:00 EST 2021

C/EBPα and GATA-2 Mutations Induce Bilineage Acute Erythroid Leukemia through Transformation of a Neomorphic Neutrophil-Erythroid Progenitor.

Cancer cell

Di Genua C,Valletta S,Buono M,Stoilova B,Sweeney C,Rodriguez-Meira A,Grover A,Drissen R,Meng Y,Beveridge R,Aboukhalil Z,Karamitros D,Belderbos ME,Bystrykh L,Thongjuea S,Vyas P,Nerlov C

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Mon May 11 00:00:00 EDT 2020

C/EBPα and GATA-2 Mutations Induce Bilineage Acute Erythroid Leukemia through Transformation of a Neomorphic Neutrophil-Erythroid Progenitor.

Cancer cell

Di Genua C,Valletta S,Buono M,Stoilova B,Sweeney C,Rodriguez-Meira A,Grover A,Drissen R,Meng Y,Beveridge R,Aboukhalil Z,Karamitros D,Belderbos ME,Bystrykh L,Thongjuea S,Vyas P,Nerlov C

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Mon May 11 00:00:00 EDT 2020

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

Snai2 Maintains Bone Marrow Niche Cells by Repressing Osteopontin Expression.

Developmental cell

Wei Q,Nakahara F,Asada N,Zhang D,Gao X,Xu C,Alfieri A,Brodin NP,Zimmerman SE,Mar JC,Guha C,Guo W,Frenette PS

45-5921-82 was used in Flow Cytometry to conclude that SNAI2 is a critical regulator of the transcriptional network maintaining MSPCs by the suppression of osteopontin expression.

Mon Jun 08 00:00:00 EDT 2020

The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Ochiai R,Matsubara E,Kondo C,Yang KM,Hoshii T,Araki M,Araki K,Sotomaru Y,Sasaki K,Mitani K,Kim DW,Ooshima A,Kim SJ

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

Methylation of a CGATA element inhibits binding and regulation by GATA-1.

Nature communications

Yang L,Chen Z,Stout ES,Delerue F,Ittner LM,Wilkins MR,Quinlan KGR,Crossley M

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Fri May 22 00:00:00 EDT 2020

A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation.

Nature communications

Kim JM,Yang YS,Park KH,Ge X,Xu R,Li N,Song M,Chun H,Bok S,Charles JF,Filhol-Cochet O,Boldyreff B,Dinter T,Yu PB,Kon N,Gu W,Takarada T,Greenblatt MB,Shim JH

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Fri May 08 00:00:00 EDT 2020

Stage Specific Expression Pattern of Alpha-Hemoglobin-Stabilizing-Protein (AHSP) Portrayed in Erythroblast Chronology.

Methods and protocols

Walczak J,Camargo Johnson MD,Muthumalaiappan K

45-5921 was used in Flow cytometry/Cell sorting to investigate gene and protein expressions of AHSP throughout progressive maturation stages of erythroblasts in biphasic cultures of blood and bone marrow samples from healthy donors.

Tue Jun 30 00:00:00 EDT 2020

Stage Specific Expression Pattern of Alpha-Hemoglobin-Stabilizing-Protein (AHSP) Portrayed in Erythroblast Chronology.

Methods and protocols

Walczak J,Camargo Johnson MD,Muthumalaiappan K

Tue Jun 30 00:00:00 EDT 2020

Aged blood impairs hippocampal neural precursor activity and activates microglia via brain endothelial cell VCAM1.

Nature medicine

Yousef H,Czupalla CJ,Lee D,Chen MB,Burke AN,Zera KA,Zandstra J,Berber E,Lehallier B,Mathur V,Nair RV,Bonanno LN,Yang AC,Peterson T,Hadeiba H,Merkel T,Körbelin J,Schwaninger M,Buckwalter MS,Quake SR,Butcher EC,Wyss-Coray T

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2019

Macrophages fine tune satellite cell fate in dystrophic skeletal muscle of mdx mice.

PLoS genetics

Madaro L,Torcinaro A,De Bardi M,Contino FF,Pelizzola M,Diaferia GR,Imeneo G,Bouchè M,Puri PL,De Santa F

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

MyD88 is required for satellite cell-mediated myofiber regeneration in dystrophin-deficient mdx mice.

Human molecular genetics

Gallot YS,Straughn AR,Bohnert KR,Xiong G,Hindi SM,Kumar A

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2018

Stem cell factor is selectively secreted by arterial endothelial cells in bone marrow.

Nature communications

Xu C,Gao X,Wei Q,Nakahara F,Zimmerman SE,Mar J,Frenette PS

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Fri Jun 22 00:00:00 EDT 2018

Age-specific biological and molecular profiling distinguishes paediatric from adult acute myeloid leukaemias.

Nature communications

Chaudhury S,O'Connor C,Cañete A,Bittencourt-Silvestre J,Sarrou E,Prendergast Á,Choi J,Johnston P,Wells CA,Gibson B,Keeshan K

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Tue Dec 11 00:00:00 EST 2018

miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling.

Nature communications

Lam J,van den Bosch M,Wegrzyn J,Parker J,Ibrahim R,Slowski K,Chang L,Martinez-Høyer S,Condorelli G,Boldin M,Deng Y,Umlandt P,Fuller M,Karsan A

45-5921 was used in Flow cytometry/Cell sorting to identify components of the transforming growth factor Β pathway as key targets of miR-143/145.

Wed Jun 20 00:00:00 EDT 2018

Collagenase-based Single Cell Isolation of Primary Murine Brain Endothelial Cells Using Flow Cytometry.

Bio-protocol

Czupalla CJ,Yousef H,Wyss-Coray T,Butcher EC

45-5921-82 was used in Flow Cytometry to report that the integrity of the brain endothelium is compromised in a plethora of neuropathologies.

Tue Nov 20 00:00:00 EST 2018

Differential cytokine contributions of perivascular haematopoietic stem cell niches.

Nature cell biology

Asada N,Kunisaki Y,Pierce H,Wang Z,Fernandez NF,Birbrair A,Ma'ayan A,Frenette PS

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

GATA4-dependent organ-specific endothelial differentiation controls liver development and embryonic hematopoiesis.

The Journal of clinical investigation

Géraud C,Koch PS,Zierow J,Klapproth K,Busch K,Olsavszky V,Leibing T,Demory A,Ulbrich F,Diett M,Singh S,Sticht C,Breitkopf-Heinlein K,Richter K,Karppinen SM,Pihlajaniemi T,Arnold B,Rodewald HR,Augustin HG,Schledzewski K,Goerdt S

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

GATA Factor-Regulated Samd14 Enhancer Confers Red Blood Cell Regeneration and Survival in Severe Anemia.

Developmental cell

Hewitt KJ,Katsumura KR,Matson DR,Devadas P,Tanimura N,Hebert AS,Coon JJ,Kim JS,Dewey CN,Keles S,Hao S,Paulson RF,Bresnick EH

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Mon Aug 07 00:00:00 EDT 2017

Engineered erythrocytes covalently linked to antigenic peptides can protect against autoimmune disease.

Proceedings of the National Academy of Sciences of the United States of America

Pishesha N,Bilate AM,Wibowo MC,Huang NJ,Li Z,Deshycka R,Bousbaine D,Li H,Patterson HC,Dougan SK,Maruyama T,Lodish HF,Ploegh HL

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Tue Mar 21 00:00:00 EDT 2017

Obesity reversibly depletes the basal cell population and enhances mammary epithelial cell estrogen receptor alpha expression and progenitor activity.

Breast cancer research : BCR

Chamberlin T,D'Amato JV,Arendt LM

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Wed Nov 29 00:00:00 EST 2017

Small molecule p300/catenin antagonist enhances hematopoietic recovery after radiation.

PloS one

Zhao Y,Wu K,Nguyen C,Smbatyan G,Melendez E,Higuchi Y,Chen Y,Kahn M

45-5921 was used in Flow cytometry/Cell sorting to report that the small molecule YH250 stimulates hematopoiesis in lethally or sublethally irradiated mice.

Tue Sep 26 00:00:00 EDT 2017

Age-dependent modulation of vascular niches for haematopoietic stem cells.

Nature

Kusumbe AP,Ramasamy SK,Itkin T,Mäe MA,Langen UH,Betsholtz C,Lapidot T,Adams RH

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Thu Apr 21 00:00:00 EDT 2016

A cell-autonomous tumour suppressor role of RAF1 in hepatocarcinogenesis.

Nature communications

Jeric I,Maurer G,Cavallo AL,Raguz J,Desideri E,Tarkowski B,Parrini M,Fischer I,Zatloukal K,Baccarini M

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Wed Dec 21 00:00:00 EST 2016

PPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal.

Nature

Lee HY,Gao X,Barrasa MI,Li H,Elmes RR,Peters LL,Lodish HF

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Thu Jun 25 00:00:00 EDT 2015

Cis-regulatory mechanisms governing stem and progenitor cell transitions.

Science advances

Johnson KD,Kong G,Gao X,Chang YI,Hewitt KJ,Sanalkumar R,Prathibha R,Ranheim EA,Dewey CN,Zhang J,Bresnick EH

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Genome-wide association study follow-up identifies cyclin A2 as a regulator of the transition through cytokinesis during terminal erythropoiesis.

American journal of hematology

Ludwig LS,Cho H,Wakabayashi A,Eng JC,Ulirsch JC,Fleming MD,Lodish HF,Sankaran VG

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Fri May 01 00:00:00 EDT 2015

Mitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis.

Cell death and differentiation

Cho J,Seo J,Lim CH,Yang L,Shiratsuchi T,Lee MH,Chowdhury RR,Kasahara H,Kim JS,Oh SP,Lee YJ,Terada N

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Heme-mediated SPI-C induction promotes monocyte differentiation into iron-recycling macrophages.

Cell

Haldar M,Kohyama M,So AY,Kc W,Wu X,Briseño CG,Satpathy AT,Kretzer NM,Arase H,Rajasekaran NS,Wang L,Egawa T,Igarashi K,Baltimore D,Murphy TL,Murphy KM

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Thu Mar 13 00:00:00 EDT 2014

CLEC-2 is required for development and maintenance of lymph nodes.

Blood

Bénézech C,Nayar S,Finney BA,Withers DR,Lowe K,Desanti GE,Marriott CL,Watson SP,Caamaño JH,Buckley CD,Barone F

45-5921 was used in Flow cytometry/Cell sorting to investigate the role that CLEC-2 plays in the development and maintenance of lymph nodes.

Thu May 15 00:00:00 EDT 2014

Concomitant inactivation of Rb and E2f8 in hematopoietic stem cells synergizes to induce severe anemia.

Blood

Hu T,Ghazaryan S,Sy C,Wiedmeyer C,Chang V,Wu L

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Thu May 10 00:00:00 EDT 2012

Gfi1b alters histone methylation at target gene promoters and sites of gamma-satellite containing heterochromatin.

The EMBO journal

Vassen L,Fiolka K,Möröy T

Published figure using TER-119 monoclonal antibody (Product # 45-5921-82) in Flow Cytometry

Wed Jun 07 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: Ly-76; Ly76; TER119

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TER-119 Monoclonal Antibody (TER-119), PerCP-Cyanine5.5, eBioscience™

TER-119 Antibody (45-5921-82) in Flow

Product Details

45-5921-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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