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14-3-3 sigma Monoclonal Antibody (3F10D9)

14-3-3 sigma Antibody in Immunocytochemistry (ICC/IF)

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14-3-3 sigma Antibody (66251-1-IG) in ICC/IF

Immunofluorescent analysis of MCF-7 cells using 66251-1-IG (14-3-3 sigma antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

14-3-3 sigma Antibody in Immunohistochemistry (Paraffin) (IHC (P))

14-3-3 sigma Antibody in Western Blot (WB)

14-3-3 sigma Antibody in Flow Cytometry (Flow)

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Product Details

66251-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:14,000

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:8000

Immunocytochemistry (ICC/IF)

1:20-1:200

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3F10D9

Immunogen

14-3-3 sigma Fusion Protein Ag12644 (1-248 aa encoded by BC002995)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.37 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MERASLIQK AKLAEQAERY EDMAAFMKGA VEKGEELSCE ERNLLSVAYK NVVGGQRAAW RVLSSIEQKS NEEGSEEKGP EVREYREKVE TELQGVCDTV LGLLDSHLIK EAGDAESRVF YLKMKGDYYR YLAEVATGDD KKRIIDSARS AYQEAMDISK KEMPPTNPIR LGLALNFSVF HYEIANSPEE AISLAKTTFD EAMADLHTLS EDSYKDSTLI MQLLRDNLTL WTADNAGEEG GEAPQEPQS (1-248 aa encoded by BC002995 )

Target Information

This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Discovery of bladder cancer biomarkers in paired pre- and postoperative urine samples.

Translational andrology and urology

Li X,Huang C,Zhang X,Yang T,Zuo S,Fu C,Zhang Y,Yang C,Chen L

Sun Aug 01 00:00:00 EDT 2021

Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis.

Nature cell biology

Kobayashi Y,Tata A,Konkimalla A,Katsura H,Lee RF,Ou J,Banovich NE,Kropski JA,Tata PR

Sat Aug 01 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: 14-3-3 protein sigma; 14-3-3 sigma; 14-3-3sigma; Epithelial cell marker protein 1; HGNC:10773; Stratifin

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Gene Aliases: HME1; SFN; YWHAS

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UniProt ID: (Human) P31947

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Entrez Gene ID: (Human) 2810

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Function(s)

protein binding protein kinase C inhibitor activity protein kinase binding protein domain specific binding phosphoprotein binding cadherin binding involved in cell-cell adhesion scaffold/adaptor protein

Process(es)

regulation of cyclin-dependent protein serine/threonine kinase activity release of cytochrome c from mitochondria keratinocyte development negative regulation of protein kinase activity DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest signal transduction intrinsic apoptotic signaling pathway in response to DNA damage regulation of epidermal cell division negative regulation of keratinocyte proliferation positive regulation of cell growth keratinization negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of epidermal cell differentiation positive regulation of protein export from nucleus membrane organization establishment of skin barrier negative regulation of protein serine/threonine kinase activity cell-cell adhesion positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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14-3-3 sigma Monoclonal Antibody (3F10D9)

14-3-3 sigma Antibody (66251-1-IG) in ICC/IF

Product Details

66251-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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