Invitrogen

alpha Actinin 4 Recombinant Rabbit Monoclonal Antibody (JU20-23)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Published Figure

3 References

View all (35) alpha Actinin 4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

alpha Actinin 4 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

alpha Actinin 4 Antibody (MA5-32794) in ICC/IF

Immunofluorescence analysis of Alpha-actinin-4 was performed using 70% confluent log phase DU 145 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with alpha Actinin 4 Recombinant Rabbit Monoclonal Antibody (JU20-23) (Product # MA5-32794) at 1:100 diluti... View More

alpha Actinin 4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

alpha Actinin 4 Antibody in Western Blot (WB)

alpha Actinin 4 Antibody in Flow Cytometry (Flow)

alpha Actinin 4 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Product Details

MA5-32794

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:100-1:500

View 1 publication 1 publication

Flow Cytometry (Flow)

1:50-1:100

Immunoprecipitation (IP)

1:10-1:50

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JU20-23

Immunogen

Synthetic peptide within Human alpha Actinin 4 aa 1-50

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2810070

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Induction of filopodia formation by α-Actinin-2 via RelA with a feedforward activation loop promoting overt bone marrow metastasis of gastric cancer.

Journal of translational medicine

Wang C,Xie B,Yin S,Cao J,Huang J,Jin L,Du G,Zhai X,Zhang R,Li S,Cao T,Yu H,Fan X,Yang Z,Peng J,Xiao J,Lian L

MA5-32794 was used in Immunocytochemistry-immunofluorescence to propose ACTN2 as a druggable molecular vulnerability that may provide potential therapeutic benefit against BMM of GC.

Mon Jun 19 00:00:00 EDT 2023

Induction of filopodia formation by α-Actinin-2 via RelA with a feedforward activation loop promoting overt bone marrow metastasis of gastric cancer.

Journal of translational medicine

Wang C,Xie B,Yin S,Cao J,Huang J,Jin L,Du G,Zhai X,Zhang R,Li S,Cao T,Yu H,Fan X,Yang Z,Peng J,Xiao J,Lian L

MA5-32794 was used in Immunocytochemistry-immunofluorescence to propose ACTN2 as a druggable molecular vulnerability that may provide potential therapeutic benefit against BMM of GC.

Mon Jun 19 00:00:00 EDT 2023

Induction of filopodia formation by α-Actinin-2 via RelA with a feedforward activation loop promoting overt bone marrow metastasis of gastric cancer.

Journal of translational medicine

Wang C,Xie B,Yin S,Cao J,Huang J,Jin L,Du G,Zhai X,Zhang R,Li S,Cao T,Yu H,Fan X,Yang Z,Peng J,Xiao J,Lian L

MA5-32794 was used in Immunocytochemistry-immunofluorescence to propose ACTN2 as a druggable molecular vulnerability that may provide potential therapeutic benefit against BMM of GC.

Mon Jun 19 00:00:00 EDT 2023

Bioinformatics

Protein Aliases: actinin-Alpha-4; alpha actinin 4; Alpha-actinin-4; F-actin cross-linking protein; focal segmental glomerulosclerosis 1; Non-muscle alpha-actinin 4

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Gene Aliases: ACTININ-4; ACTN4; C77391; FSGS; FSGS1

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UniProt ID: (Human) O43707, (Mouse) P57780, (Rat) Q9QXQ0

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Entrez Gene ID: (Human) 81, (Mouse) 60595, (Rat) 63836

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding nucleoside binding actin binding integrin binding calcium ion binding protein binding ligand-dependent nuclear receptor transcription coactivator activity chromatin DNA binding nuclear hormone receptor binding protein homodimerization activity retinoic acid receptor binding ion channel binding poly(A) RNA binding protein N-terminus binding actin filament binding protein complex binding metal ion binding

Process(es)

transport protein transport peroxisome proliferator activated receptor signaling pathway regulation of apoptotic process retinoic acid receptor signaling pathway positive regulation of pinocytosis actin filament bundle assembly negative regulation of cellular component movement positive regulation of cellular component movement bicellular tight junction assembly protein localization to bicellular tight junction regulation of nucleic acid-templated transcription response to hypoxia platelet degranulation vesicle transport along actin filament positive regulation of cell migration positive regulation of sodium:proton antiporter activity negative regulation of substrate adhesion-dependent cell spreading positive regulation of NIK/NF-kappaB signaling

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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alpha Actinin 4 Recombinant Rabbit Monoclonal Antibody (JU20-23)

alpha Actinin 4 Antibody (MA5-32794) in ICC/IF

Product Details

MA5-32794

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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