Invitrogen

p38 MAPK gamma Monoclonal Antibody (10E1)

p38 MAPK gamma Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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p38 MAPK gamma Antibody (MA1-100) in IHC (P)

Immunohistochemistry analysis of MAPK12 showing positive staining in the nucleus and cytoplasm of paraffin-treated Human breast carcinoma (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-... View More

p38 MAPK gamma Antibody in Western Blot (WB)

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Product Details

MA1-100

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Product Specifications

Species Reactivity

Dog, Human, Mouse

Host/Isotype

Mouse / IgG

Class

Monoclonal

Type

Antibody

Clone

10E1

Immunogen

Protein expressed in 293T cell transfected with human MAPK12 expression vector

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536744

Product Specific Information

MA1-100 has been successfully used in Western blot and immunohistochemistry applications and reacts with human and mouse samples.

MA1-100 specifically recognizes p38-gamma, and does not react with alpha, beta, or delta isoforms.

Target Information

MAPK12 (p38 gamma) is a serine/threonine kinase involved in many different cellular processes. It is activated during hypoxia and gamma-irradiation-induced G(2) arrest. Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. MAPK12 functions as a signal transducer during differentiation of myoblasts to myotubes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ERK-6; ERK5; Extracellular signal-regulated kinase 6; MAP kinase 12; MAP kinase p38 gamma; MAPK 12; mitogen activated protein kinase 12; Mitogen-activated protein kinase 12; mitogen-activated protein kinase 3; Mitogen-activated protein kinase p38 gamma; OTTHUMP00000196671; stress activated protein kinase 3; Stress-activated protein kinase 3

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Gene Aliases: AW123708; ERK-6; ERK3; ERK6; MAPK 12; MAPK12; P38GAMMA; PRKM12; SAPK-3; SAPK3

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UniProt ID: (Human) P53778, (Mouse) O08911

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Entrez Gene ID: (Human) 6300, (Dog) 607023, (Mouse) 29857

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Function(s)

magnesium ion binding protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups metal ion binding non-receptor serine/threonine protein kinase

Process(es)

MAPK cascade transcription, DNA-templated regulation of transcription, DNA-templated DNA damage induced protein phosphorylation cell cycle arrest signal transduction muscle organ development positive regulation of peptidase activity peptidyl-serine phosphorylation myoblast differentiation vascular endothelial growth factor receptor signaling pathway positive regulation of muscle cell differentiation protein phosphorylation cell cycle phosphorylation negative regulation of cell cycle

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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p38 MAPK gamma Monoclonal Antibody (10E1)

p38 MAPK gamma Antibody (MA1-100) in IHC (P)

Product Details

MA1-100

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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