Invitrogen

MCP-1 Polyclonal Antibody, PeproTech®

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MCP-1 Antibody (500-P76-1MG) in WB

Western Blot: To detect Rat MCP-1(MCAF) by Western Blot analysis MCP-1 Polyclonal Antibody (Product # 500-P76-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat MCP-1(MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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Product Details

500-P76-1MG

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

Immunohistochemistry (IHC)

View 3 publications 3 publications

ELISA (ELISA)

0.5-2.0 µg/mL

View 4 publications 4 publications

Neutralization (Neu)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Immunostaining (IS)

View 5 publications 5 publications

Product Specifications

Species Reactivity

Rat

Published species

Pig, Rat

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Rat MCP-1 (CCL2)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930750

Product Specific Information

AA Sequence of recombinant protein: QPDAVNAPLT CCYSFTGKMI PMSRLENYKR ITSSRCPKEA VVFVTKLKRE ICADPNKEWV QKYIRKLDQN QVRSETTVFY KIASTLRTSA PLNVNLTHKS EANASTLFST TTSSTSVEVT SMTEN.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat MCP-1 (CCL2). Anti-Rat MCP-1 (CCL2)-specific antibody was purified by affinity chromatography employing an immobilized Rat MCP-1 (CCL2) matrix.

Sandwich ELISA: To detect Rat MCP-1 (CCL2) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat MCP-1 (CCL2) (500-P76Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat MCP-1 (CCL2).

Western Blot: To detect Rat MCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat MCP-1(MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Activities of Ca²⁺-related ion channels during the formation of kidney stones in an infection-induced urolithiasis rat model.

American journal of physiology. Renal physiology

Cherng JH,Hsu YJ,Liu CC,Tang SH,Sartika D,Chang SJ,Fan GY,Wu ST,Meng E

500-P76 was used in Immunohistochemistry to study the correlation between infection-promoted CaOx kidney stones and alterations in Ca2-related ion channels using a rat model.

Fri Nov 01 00:00:00 EDT 2019

Epothilone B impairs functional recovery after spinal cord injury by increasing secretion of macrophage colony-stimulating factor.

Cell death & disease

Mao L,Gao W,Chen S,Song Y,Song C,Zhou Z,Zhao H,Zhou K,Wang W,Zhu K,Liu C,Mei X

Thu Nov 02 00:00:00 EDT 2017

Epicardial adipose excision slows the progression of porcine coronary atherosclerosis.

Journal of cardiothoracic surgery

McKenney ML,Schultz KA,Boyd JH,Byrd JP,Alloosh M,Teague SD,Arce-Esquivel AA,Fain JN,Laughlin MH,Sacks HS,Sturek M

500-P76 was used in Immunohistochemistry to evaluate the excision of epicardial adipose tissue and its effects on coronary atherosclerosis in pigs.

Fri Jan 03 00:00:00 EST 2014

Systemic immunotherapy delays photoreceptor cell loss and prevents vascular pathology in Royal College of Surgeons rats.

Molecular vision

Adamus G,Wang S,Kyger M,Worley A,Lu B,Burrows GG

Tue Jan 29 00:00:00 EST 2013

Trans- but not cis-resveratrol impairs angiotensin-II-mediated vascular inflammation through inhibition of NF-κB activation and peroxisome proliferator-activated receptor-gamma upregulation.

Journal of immunology (Baltimore, Md. : 1950)

Rius C,Abu-Taha M,Hermenegildo C,Piqueras L,Cerda-Nicolas JM,Issekutz AC,Estañ L,Cortijo J,Morcillo EJ,Orallo F,Sanz MJ

Wed Sep 15 00:00:00 EDT 2010

Type V collagen-induced oral tolerance plus low-dose cyclosporine prevents rejection of MHC class I and II incompatible lung allografts.

Journal of immunology (Baltimore, Md. : 1950)

Yamada Y,Sekine Y,Yoshida S,Yasufuku K,Petrache I,Benson HL,Brand DD,Yoshino I,Wilkes DS

Wed Jul 01 00:00:00 EDT 2009

Menopause and ovariectomy cause a low grade of systemic inflammation that may be prevented by chronic treatment with low doses of estrogen or losartan.

Journal of immunology (Baltimore, Md. : 1950)

Abu-Taha M,Rius C,Hermenegildo C,Noguera I,Cerda-Nicolas JM,Issekutz AC,Jose PJ,Cortijo J,Morcillo EJ,Sanz MJ

Wed Jul 15 00:00:00 EDT 2009

Force-dependent development of neuropathic central pain and time-related CCL2/CCR2 expression after graded spinal cord contusion injuries of the rat.

Journal of neurotrauma

Knerlich-Lukoschus F,Juraschek M,Blömer U,Lucius R,Mehdorn HM,Held-Feindt J

Thu May 01 00:00:00 EDT 2008

A critical role for TNFalpha in the selective attachment of mononuclear leukocytes to angiotensin-II-stimulated arterioles.

Blood

Mateo T,Naim Abu Nabah Y,Losada M,Estellés R,Company C,Bedrina B,Cerdá-Nicolás JM,Poole S,Jose PJ,Cortijo J,Morcillo EJ,Sanz MJ

Sat Sep 15 00:00:00 EDT 2007

Angiotensin II-induced mononuclear leukocyte interactions with arteriolar and venular endothelium are mediated by the release of different CC chemokines.

Journal of immunology (Baltimore, Md. : 1950)

Mateo T,Abu Nabah YN,Abu Taha M,Mata M,Cerdá-Nicolás M,Proudfoot AE,Stahl RA,Issekutz AC,Cortijo J,Morcillo EJ,Jose PJ,Sanz MJ

Mon May 01 00:00:00 EDT 2006

Antisense to transforming growth factor-beta1 messenger RNA reduces vein graft intimal hyperplasia and monocyte chemotactic protein 1.

Journal of vascular surgery

Wolff RA,Ryomoto M,Stark VE,Malinowski R,Tomas JJ,Stinauer MA,Hullett DA,Hoch JR

500-P76 was used in Enzyme-linked immunosorbent assay to demonstrate that perioperative antisense TGF-beta1 treatment of the vein to be used in arterial reconstructions resulted in a prolonged diminution of intimal hyperplasia (IH).

Tue Mar 01 00:00:00 EST 2005

Antisense to transforming growth factor-beta1 messenger RNA reduces vein graft intimal hyperplasia and monocyte chemotactic protein 1.

Journal of vascular surgery

Wolff RA,Ryomoto M,Stark VE,Malinowski R,Tomas JJ,Stinauer MA,Hullett DA,Hoch JR

Tue Mar 01 00:00:00 EST 2005

Expression of monocyte chemoattractant protein-1 in proximal tubular epithelial cells in a rat model of progressive kidney failure.

The Journal of laboratory and clinical medicine

Ota T,Tamura M,Osajima A,Doi Y,Kudo H,Anai H,Miyazaki M,Nishino T,Nakashima Y

Mon Jul 01 00:00:00 EDT 2002

Angiotensin II induces monocyte chemoattractant protein-1 gene expression in rat vascular smooth muscle cells.

Circulation research

Chen XL,Tummala PE,Olbrych MT,Alexander RW,Medford RM

Mon Nov 02 00:00:00 EST 1998

Bioinformatics

Protein Aliases: C-C motif chemokine 2; H-MCP-1; immediate-early serum-responsive JE protein; Immediate-early serum-responsive protein JE; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic protein 1; RP23-350G1.3; small inducible cytokine A2; small inducible gene JE; Small-inducible cytokine A2

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Gene Aliases: Ccl2; Je; MCP-1; Mcp1; Scya2; Sigje

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UniProt ID: (Rat) P14844

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Entrez Gene ID: (Rat) 24770

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Function(s)

G-protein coupled receptor binding chemokine activity heparin binding CCR2 chemokine receptor binding cytokine intercellular signal molecule

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MCP-1 Polyclonal Antibody, PeproTech®

MCP-1 Antibody (500-P76-1MG) in WB

Product Details

500-P76-1MG

Western Blot (WB)

Immunohistochemistry (IHC)

ELISA (ELISA)

Neutralization (Neu)

Miscellaneous PubMed (Misc)

Immunostaining (IS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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