Invitrogen

K-Ras Monoclonal Antibody (A8E5)

K-Ras Antibody in Immunocytochemistry (ICC/IF)

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K-Ras Antibody (MA5-50602) in ICC/IF

Immunocytochemistry analysis of K-Ras in NIH/3T3 cells. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were incubated with K-Ras monoclonal antibody (Product # MA5-50602) with a dilution of 1:100 in 2% BSA overnight at 4 . Followed by secondary antibody Goat Anti-Mouse IgG H&L (iFluor™ 488) at a dilution of 1:1,000. PBS instead of the primary antibody was used as the secondary antibody only control. Nu... View More

K-Ras Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

MA5-50602

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:1,100

Immunocytochemistry (ICC/IF)

1:100

Flow Cytometry (Flow)

1:500-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

A8E5

Immunogen

Recombinant protein within human KRAS aa 2-186/189.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3093988

Product Specific Information

Positive control: Rat brain tissue lysate, PC-12 cell lysate, human kidney tissue lysate, Jurkat cell lysate, NIH/3T3, human lung carcinoma tissue, Hela.

Predicted band size: 21 kDa

Subcellular Location: Cell membrane Cytoplasm.

Target Information

K-RAS (GTPase KRas) belongs to the Ras oncogene family. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. It plays an important role in the regulation of cell proliferation and has a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes in colorectal cancer cells in a ZNF304-dependent manner. Mutations in the gene can result in leukemia acute myelogenous, leukemia juvenile myelomonocytic, Noonan Syndrome, gastric cancer, and cardiofaciocutaneous syndrome 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: c-K-ras; c-K-ras2 protein; c-Ki-ras; c-Kirsten-ras protein; c-Kirsten-ras proto-oncogene; cellular c-Ki-ras2; cellular c-Ki-ras2 proto-oncogene; GTPase KRas; K RAS; K-Ras 2; K-ras p21 protein; Ki-Ras; Kirsten rat sarcoma oncogene 2, expressed; Kirsten rat sarcoma viral oncogene homolog; Kirsten rat sarcoma viral oncogene homologue 2 (active); oncogene KRAS2; p21 protein; PR310 c-K-ras oncogene; RASK; transforming protein p21; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog; v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

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Gene Aliases: AI929937; C-K-RAS; c-Ki-ras; CFC2; K-ras; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS; Kras-2; KRAS1; KRAS2; NS; NS3; p21; p21B; RALD; ras; RASK2

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UniProt ID: (Human) P01116, (Rat) P08644, (Mouse) P32883

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Entrez Gene ID: (Human) 3845, (Rat) 24525, (Mouse) 16653

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Function(s)

GTPase activity protein binding GTP binding GMP binding GDP binding LRR domain binding protein complex binding nucleotide binding small GTPase

Process(es)

MAPK cascade positive regulation of protein phosphorylation stimulatory C-type lectin receptor signaling pathway epidermal growth factor receptor signaling pathway Ras protein signal transduction axon guidance positive regulation of cell proliferation visual learning positive regulation of gene expression cytokine-mediated signaling pathway forebrain astrocyte development actin cytoskeleton organization regulation of protein stability regulation of synaptic transmission, GABAergic positive regulation of Rac protein signal transduction social behavior endocrine signaling Fc-epsilon receptor signaling pathway ERBB2 signaling pathway positive regulation of MAP kinase activity negative regulation of neuron apoptotic process negative regulation of cell differentiation regulation of long-term neuronal synaptic plasticity homeostasis of number of cells within a tissue leukocyte migration positive regulation of nitric-oxide synthase activity positive regulation of NF-kappaB transcription factor activity striated muscle cell differentiation response to glucocorticoid response to mineralocorticoid epithelial tube branching involved in lung morphogenesis small GTPase mediated signal transduction signal transduction

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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K-Ras Monoclonal Antibody (A8E5)

K-Ras Antibody (MA5-50602) in ICC/IF

Product Details

MA5-50602

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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