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CD34 Monoclonal Antibody (581), FITC

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CD34 Antibody (CD34-581-01) in Flow

Experimental and therapeutic medicine 2021 - Figure 1 Culture and identification of bone marrow mesenchymal stem cells. (A) Light microscopy of BMSCs (scale bar=100 um for the left image and 200 um for the right image). (B) Percentage of CD34-, CD45-, CD73-, CD90- and CD105-positive BMSCs were detected by flow cytometry. BMSC, bone marrow mesenchymal stem cell.

CD34 Antibody in Immunohistochemistry (IHC)

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Product Details

CD34-581-01

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL for 1x10^6 cells

View 14 publications 14 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

581

Immunogen

Human CD34.

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

purified

Storage buffer

PBS with 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536499

Target Information

CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

TNF-α activates RELA expression via TNFRSF1B to upregulate OPA1 expression and inhibit chondrogenic differentiation of human adipose stem cells.

Journal of orthopaedic surgery and research

Guo J,Ye W,Wu X,Huang H,Li B,Sun Z,Ren Z,Yang Z

CD34-581-01 was used in Flow cytometry/Cell sorting to show that the presence of TNF-α led to increased expression of TNFα receptor 2 (TNFRSF1B) and RELA during chondrogenic differentiation of hADSCs.

Tue Jun 13 00:00:00 EDT 2023

Bcl-xL mutant promotes cartilage differentiation of BMSCs by upregulating TGF-β/BMP expression levels.

Experimental and therapeutic medicine

Xiao K,Yang L,Xie W,Gao X,Huang R,Xie M

CD34-581-01 was used in Flow Cytometry to investigate the impact of this Bcl-xL mutant on cartilage differentiation and the expression levels of TGF-β and bone morphogenetic protein (BMP).

Thu Jul 01 00:00:00 EDT 2021

Bcl-xL mutant promotes cartilage differentiation of BMSCs by upregulating TGF-β/BMP expression levels.

Experimental and therapeutic medicine

Xiao K,Yang L,Xie W,Gao X,Huang R,Xie M

CD34-581-01 was used in Flow Cytometry to investigate the impact of this Bcl-xL mutant on cartilage differentiation and the expression levels of TGF-β and bone morphogenetic protein (BMP).

Thu Jul 01 00:00:00 EDT 2021

Isolation of Multipotent Mesenchymal Stem Cells from Human Extraocular Muscle Tissue.

Bio-protocol

Sharma A,Mawrie D,Magdalene D,Jaganathan BG

CD34-581-01 was used in Flow Cytometry to explore extra-ocular muscle tissues obtained from patients as a novel source of mesenchymal stem cells (MSCs) which express characteristic cell surface markers of MSCs and show multilineage differentiation potential with high proliferation capacity.

Wed Feb 20 00:00:00 EST 2019

Impact of Tissue Harvesting Sites on the Cellular Behaviors of Adipose-Derived Stem Cells: Implication for Bone Tissue Engineering.

Stem cells international

Rezai Rad M,Bohloli M,Akhavan Rahnama M,Anbarlou A,Nazeman P,Khojasteh A

CD34-581-01 was used in Flow cytometry/Cell sorting to quantitatively compare the proliferation and osteogenic capabilities of adipose-derived stem cells from different harvesting sites.

Tue Sep 29 00:00:00 EDT 2020

Oxysterols in adipose tissue-derived mesenchymal stem cell proliferation and death.

The Journal of steroid biochemistry and molecular biology

Silva SF,Levy D,Ruiz JLM,de Melo TC,Isaac C,Fidelis ML,Rodrigues A,Bydlowski SP

CD34-581-01 was used in flow cytometry to research adipose tissue-derived mesenchymal stem cell proliferation and death with oxysterols

Mon May 01 00:00:00 EDT 2017

Analysis of the safety of mesenchymal stromal cells secretome for glioblastoma treatment.

Cytotherapy

Onzi GR,Ledur PF,Hainzenreder LD,Bertoni AP,Silva AO,Lenz G,Wink MR

CD3458101 was used in flow cytometry to assess the use of human adipose-derived stem cells to treat cancer

Fri Jul 01 00:00:00 EDT 2016

Circulating precursors of human CD1c+ and CD141+ dendritic cells.

The Journal of experimental medicine

Breton G,Lee J,Zhou YJ,Schreiber JJ,Keler T,Puhr S,Anandasabapathy N,Schlesinger S,Caskey M,Liu K,Nussenzweig MC

Published figure using CD34 monoclonal antibody (Product # CD34-581-01) in Flow Cytometry

Mon Mar 09 00:00:00 EDT 2015

Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow.

The Journal of experimental medicine

Lee J,Breton G,Oliveira TY,Zhou YJ,Aljoufi A,Puhr S,Cameron MJ,Sékaly RP,Nussenzweig MC,Liu K

Published figure using CD34 monoclonal antibody (Product # CD34-581-01) in Flow Cytometry

Mon Mar 09 00:00:00 EDT 2015

Mesenchymal stem cells augment the anti-bacterial activity of neutrophil granulocytes.

PloS one

Brandau S,Jakob M,Bruderek K,Bootz F,Giebel B,Radtke S,Mauel K,Jäger M,Flohé SB,Lang S

CD34-581-01 was used in flow cytometry to study the effect of mesenchymal stem cells on the anti-bacterial activity of neutrophil granulocytes

Tue Jun 16 00:00:00 EDT 2015

The bidirectional tumor--mesenchymal stromal cell interaction promotes the progression of head and neck cancer.

Stem cell research & therapy

Kansy BA,Dißmann PA,Hemeda H,Bruderek K,Westerkamp AM,Jagalski V,Schuler P,Kansy K,Lang S,Dumitru CA,Brandau S

CD34-581-01 was used in flow cytometry to isolate and characterize mesenchymal stromal cells from head and neck squamous cell carcinoma and study their interaction with tumor cells

Tue Aug 12 00:00:00 EDT 2014

Impaired immunomodulatory ability of bone marrow mesenchymal stem cells on CD4(+) T cells in aplastic anemia.

Results in immunology

Li J,Lu S,Yang S,Xing W,Feng J,Li W,Zhao Q,Wu H,Ge M,Ma F,Zhao H,Liu B,Zhang L,Zheng Y,Han ZC

Published figure using CD34 monoclonal antibody (Product # CD34-581-01) in Flow Cytometry

Fri Dec 27 00:00:00 EST 2013

Assessment of human multi-potent hematopoietic stem/progenitor cell potential using a single in vitro screening system.

PloS one

Calvo J,BenYoucef A,Baijer J,Rouyez MC,Pflumio F

Published figure using CD34 monoclonal antibody (Product # CD34-581-01) in Flow Cytometry

Wed May 22 00:00:00 EDT 2013

Restoration of cellular function of mesenchymal stem cells from a hypophosphatasia patient.

Gene therapy

Katsube Y,Kotobuki N,Tadokoro M,Kanai R,Taketani T,Yamaguchi S,Ohgushi H

CD34-581-01 was used in Flow Cytometry to use autologous MSCs for the treatment of hypophosphatasia.

Thu Apr 01 00:00:00 EDT 2010

Phyllodes tumor of the seminal vesicle: case report and literature review.

Pathology international

Son HJ,Jeong YJ,Kim JH,Chung MJ

Published figure using CD34 monoclonal antibody (Product # CD34-581-01) in Immunohistochemistry

Wed Dec 01 00:00:00 EST 2004

Antigen CD34+ marrow cells engraft lethally irradiated baboons.

The Journal of clinical investigation

Berenson RJ,Andrews RG,Bensinger WI,Kalamasz D,Knitter G,Buckner CD,Bernstein ID

CD34-581-01 was used in flow cytometry to propose that stem cells responsible for hematopoietic reconstitution are CD34+

Tue Mar 01 00:00:00 EST 1988

Bioinformatics

Protein Aliases: CD34; CD34 antigen; Hematopoietic progenitor cell antigen CD34; Mucosialin; RP11-328D5.2

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Gene Aliases: CD34

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UniProt ID: (Human) P28906

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Entrez Gene ID: (Human) 947

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Function(s)

transcription factor binding carbohydrate binding sulfate binding cell adhesion molecule

Process(es)

tissue homeostasis endothelial cell proliferation glomerular filtration endothelium development cell-matrix adhesion signal transduction regulation of blood pressure cell proliferation positive regulation of gene expression negative regulation of gene expression single organismal cell-cell adhesion hemopoiesis negative regulation of blood coagulation negative regulation of tumor necrosis factor production positive regulation of interleukin-10 production mesangial cell-matrix adhesion paracrine signaling positive regulation of odontogenesis negative regulation of nitric oxide biosynthetic process positive regulation of angiogenesis cell motility regulation of immune response leukocyte migration transdifferentiation vascular wound healing hematopoietic stem cell proliferation positive regulation of transforming growth factor beta production positive regulation of granulocyte colony-stimulating factor production extracellular exosome assembly glomerular endothelium development stem cell proliferation metanephric glomerular mesangial cell differentiation negative regulation of cellular response to heat negative regulation of cellular response to hypoxia negative regulation of interleukin-2 secretion positive regulation of glial cell-derived neurotrophic factor secretion negative regulation of neuron death positive regulation of vasculogenesis

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD34 Monoclonal Antibody (581), FITC

CD34 Antibody (CD34-581-01) in Flow

Product Details

CD34-581-01

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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