Invitrogen

CD34 Polyclonal Antibody

CD34 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

CD34 Antibody (PA5-85917) in ICC/IF

Immunocytochemical analysis of CD34 in hES cells using a CD34 Polyclonal antibody (Product # PA5-85917) as seen in green. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

CD34 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

PA5-85917

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:2,000-1:3,000

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:250

Flow Cytometry (Flow)

1:1,000

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide within Human CD34 aa 336-385

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 40% Glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2802718

Target Information

CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Extracellular vesicles from bone marrow mesenchymal stem cells alleviate acute rejection injury after liver transplantation by carrying miR-22-3p and inducing M2 polarization of Kupffer cells.

The journal of gene medicine

Zhou M,Hui J,Gao L,Liang J,Wang C,Xu J

Published figure using CD34 polyclonal antibody (Product # PA5-85917) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2023

Therapeutic role of adipose-derived mesenchymal stem cells-derived extracellular vesicles in rats with obstructive sleep apnea hypopnea syndrome.

Regenerative therapy

Xu L,Zhang L,Xiang Y,Zhang X

PA5-85917 was used in Flow cytometry/Cell sorting to find that ADSCs-EVs transferred miR-22-3p to pneumonocytes and reduced apoptosis, inflammation, and oxidative stress through KDM6B/HMGA2, mitigating OSAHS-LI progression.

Wed Mar 01 00:00:00 EST 2023

Therapeutic role of adipose-derived mesenchymal stem cells-derived extracellular vesicles in rats with obstructive sleep apnea hypopnea syndrome.

Regenerative therapy

Xu L,Zhang L,Xiang Y,Zhang X

Wed Mar 01 00:00:00 EST 2023

Propofol suppresses adipose-derived stem cell progression via PI3K/AKT-Wnt signaling pathway.

BMC anesthesiology

Yin G,Wang J,Zhong Y,Wu W

PA5-85917 was used in Flow Cytometry to indicate that the PI3K/AKT-Wnt pathways mediated propofol-inhibited ADSC proliferation, providing new insights into the propofol application in ADSCs.

Wed Mar 09 00:00:00 EST 2022

Propofol suppresses adipose-derived stem cell progression via PI3K/AKT-Wnt signaling pathway.

BMC anesthesiology

Yin G,Wang J,Zhong Y,Wu W

PA5-85917 was used in Flow Cytometry to indicate that the PI3K/AKT-Wnt pathways mediated propofol-inhibited ADSC proliferation, providing new insights into the propofol application in ADSCs.

Wed Mar 09 00:00:00 EST 2022

Fully Reduced HMGB1-Containing Peptide-Based Polyurethane Scaffold with Minimal Functional Unit of Skin (MFUS) Enhances Large and Deep Wounded Skin Healing.

Macromolecular bioscience

Chang P,Li S,Sun Q,Guo K,Wang H,Li S,Zhang L,Xie Y,Zheng X,Liu Y

PA5-85917 was used in Immunohistochemistry to demonstrate that the combination of fully reduced HMGB1-LDI-Gly polymer with MFUS provides a new tissue engineering approach for large full-thickness skin wound healing.

Tue Feb 01 00:00:00 EST 2022

Over-expression of MEG3 promotes differentiation of bone marrow mesenchymal stem cells into chondrocytes by regulating miR-129-5p/RUNX1 axis.

Cell cycle (Georgetown, Tex.)

Zhu J,Fu Q,Shao J,Peng J,Qian Q,Zhou Y,Chen Y

PA5-85917 was used in Flow Cytometry to explored the effect and mechanism of MEG3 on chondrocyte differentiation under the induction of bone marrow mesenchymal stem cells.

Fri Jan 01 00:00:00 EST 2021

Over-expression of MEG3 promotes differentiation of bone marrow mesenchymal stem cells into chondrocytes by regulating miR-129-5p/RUNX1 axis.

Cell cycle (Georgetown, Tex.)

Zhu J,Fu Q,Shao J,Peng J,Qian Q,Zhou Y,Chen Y

PA5-85917 was used in Flow Cytometry to explored the effect and mechanism of MEG3 on chondrocyte differentiation under the induction of bone marrow mesenchymal stem cells.

Fri Jan 01 00:00:00 EST 2021

Aquatic Training after Joint Immobilization in Rats Promotes Adaptations in Myotendinous Junctions.

International journal of molecular sciences

Rocha LC,Barbosa GK,Pimentel Neto J,Jacob CDS,Knudsen AB,Watanabe IS,Ciena AP

PA5-85917 was used in Immunohistochemistry to investigate the components of the myotendinous junction and their adaptations and the associated elements triggered with aquatic training after joint immobilization.

Tue Jun 29 00:00:00 EDT 2021

Myotendinous junction adaptations to ladder-based resistance training: identification of a new telocyte niche.

Scientific reports

Pimentel Neto J,Rocha LC,Barbosa GK,Jacob CDS,Krause Neto W,Watanabe IS,Ciena AP

PA5-85917 was used in Immunocytochemistry to conclude that the continuous adjustments in the MTJ ultrastructure are the result of tissue plasticity induced by LRT, which is causally related to muscle hypertrophy and, consequently, to the remodeling of the contact interface.

Mon Aug 24 00:00:00 EDT 2020

Myotendinous junction adaptations to ladder-based resistance training: identification of a new telocyte niche.

Scientific reports

Pimentel Neto J,Rocha LC,Barbosa GK,Jacob CDS,Krause Neto W,Watanabe IS,Ciena AP

Mon Aug 24 00:00:00 EDT 2020

Bone marrow mesenchymal stem cell-derived exosomes protect against myocardial infarction by promoting autophagy.

Experimental and therapeutic medicine

Zou L,Ma X,Lin S,Wu B,Chen Y,Peng C

PA5-85917 was used in Flow cytometry/Cell sorting to investigate the potential therapeutic effects of MSC exosomes on myocardial injuries that are caused by myocardial infarction.

Tue Oct 01 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: CD34; CD34 antigen; cluster designation 34; Hematopoietic progenitor cell antigen CD34; Mucosialin; RP11-328D5.2

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Gene Aliases: AU040960; CD34

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UniProt ID: (Human) P28906, (Mouse) Q64314

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Entrez Gene ID: (Human) 947, (Rat) 305081, (Mouse) 12490

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Function(s)

transcription factor binding carbohydrate binding sulfate binding cell adhesion molecule

Process(es)

tissue homeostasis endothelial cell proliferation glomerular filtration endothelium development cell-matrix adhesion signal transduction regulation of blood pressure cell proliferation positive regulation of gene expression negative regulation of gene expression single organismal cell-cell adhesion hemopoiesis negative regulation of blood coagulation negative regulation of tumor necrosis factor production positive regulation of interleukin-10 production mesangial cell-matrix adhesion paracrine signaling positive regulation of odontogenesis negative regulation of nitric oxide biosynthetic process positive regulation of angiogenesis cell motility regulation of immune response leukocyte migration transdifferentiation vascular wound healing hematopoietic stem cell proliferation positive regulation of transforming growth factor beta production positive regulation of granulocyte colony-stimulating factor production extracellular exosome assembly glomerular endothelium development stem cell proliferation metanephric glomerular mesangial cell differentiation negative regulation of cellular response to heat negative regulation of cellular response to hypoxia negative regulation of interleukin-2 secretion positive regulation of glial cell-derived neurotrophic factor secretion negative regulation of neuron death positive regulation of vasculogenesis cell adhesion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD34 Polyclonal Antibody

CD34 Antibody (PA5-85917) in ICC/IF

Product Details

PA5-85917

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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